Wu Guannan, Zhao Ming, Gu Xiaoling, Yao Yanwen, Liu Hongbing, Song Yong
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, Jiangsu Province 210002, PR China.
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, Jiangsu Province 210002, PR China.
Infect Genet Evol. 2014 Jun;24:82-91. doi: 10.1016/j.meegid.2014.03.006. Epub 2014 Mar 17.
Studies of the association between the purinergic receptor P2X, ligand-gated ion channel 7 (P2X7 receptor) gene 1513A/C polymorphism and susceptibility to tuberculosis have yielded inconsistent results. We performed this meta-analysis to help clarify these inconsistencies. After systematically searching PUBMED, MEDLINE, EMBASE and ISI Web of knowledge, two of the authors independently extracted relevant data and a meta-analysis was performed by using STATA11.0 software. A total number of nine studies involving 2195 cases and 2036 controls were identified. The results indicated that P2X7 receptor gene 1513C allele (OR 1.389, 95% CI 1.161-1.660, p<0.001) and CC genotype (1.582, 95% CI 1.129-2.217, p=0. 012) were significantly associated with increased susceptibility to tuberculosis. Subgroup analysis indicated that this SNP greatly contributed to susceptibility to tuberculosis in Asians. The C allele of P2X7 receptor gene 1513A/C polymorphism was also associated with increased susceptibility to pulmonary tuberculosis in Asians (C vs. A: OR 1.420, 95% CI 1.163-1.733, p=0.001; (CC+AC) vs. AA: OR 1.522, 95% CI 1.186-1.953, p=0.001). Greater association between P2X7 receptor gene 1513A/C polymorphism and susceptibility to extra-pulmonary tuberculosis with bigger ORs were found (C vs. A, OR 2.035, 95% CI 1.236-3.352, p=0.005; CC vs. AA, OR 3.788, 95% CI 1.434-10.009, p=0.007; AC vs. AA, OR 2.148, 95% CI 1.252-3.684, p=0.005; (CC+AC) vs. AA, OR 2.386, 95% CI 1.302-4.374, p=0.005; CC vs. (AC+AA), OR 2.692, 95% CI 1.242-5.836, p=0. 012). This meta-analysis indicates that the C allele of P2X7 receptor gene 1513A/C polymorphism is a risk factor for pulmonary tuberculosis in Asians, while not in Africans or Latinos and a risk for extra-pulmonary tuberculosis. Further well-designed, large scale studies are required to confirm this conclusion.
关于嘌呤能受体P2X、配体门控离子通道7（P2X7受体）基因1513A/C多态性与结核病易感性之间关联的研究结果并不一致。我们进行了这项荟萃分析以帮助阐明这些不一致之处。在系统检索了PUBMED、MEDLINE、EMBASE和ISI Web of knowledge之后，两位作者独立提取相关数据，并使用STATA11.0软件进行荟萃分析。共确定了9项研究，涉及2195例病例和2036例对照。结果表明，P2X7受体基因1513C等位基因（比值比1.389，95%置信区间1.161 - 1.660，p<0.001）和CC基因型（1.582，95%置信区间1.129 - 2.217，p = 0.012）与结核病易感性增加显著相关。亚组分析表明，该单核苷酸多态性对亚洲人结核病易感性有很大影响。P2X7受体基因1513A/C多态性的C等位基因在亚洲人中也与肺结核易感性增加相关（C与A相比：比值比1.420，95%置信区间1.163 - 1.733，p = 0.001；(CC + AC)与AA相比：比值比1.522，95%置信区间1.186 - 1.953，p = 0.001）。发现P2X7受体基因1513A/C多态性与肺外结核易感性之间存在更强的关联，比值比更大（C与A相比，比值比2.035，95%置信区间1.236 - 3.352，p = 0.005；CC与AA相比，比值比3.788，95%置信区间1.434 - 10.009，p = 0.007；AC与AA相比，比值比2.148，95%置信区间1.252 - 3.684，p = 0.005；(CC + AC)与AA相比，比值比2.386，95%置信区间1.302 - 4.374，p = 0.005；CC与(AC + AA)相比，比值比2.692，95%置信区间1.242 - 5.836，p = 0.012）。这项荟萃分析表明，P2X7受体基因1513A/C多态性的C等位基因是亚洲人患肺结核的一个危险因素，而在非洲人或拉丁美洲人中并非如此，并且是肺外结核的一个危险因素。需要进一步设计良好的大规模研究来证实这一结论。
