Dreisewerd Klaus
Institute for Hygiene, Biomedical Mass Spectrometry, University of Münster, Robert-Koch-Str. 41, 48149, Münster, Germany,
Anal Bioanal Chem. 2014 Apr;406(9-10):2261-78. doi: 10.1007/s00216-014-7646-6. Epub 2014 Mar 21.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for characterization of large, thermally labile biomolecules. Advantages of this analytical technique are high sensitivity, robustness, high-throughput capacity, and applicability to a wide range of compound classes. For some years, MALDI-MS has also been increasingly used for mass spectrometric imaging as well as in other areas of clinical research. Recently, several new concepts have been presented that have the potential to further advance the performance characteristics of MALDI. Among these innovations are novel matrices with low proton affinities for particularly efficient protonation of analyte molecules, use of wavelength-tunable lasers to achieve optimum excitation conditions, and use of liquid matrices for improved quantification. Instrumental modifications have also made possible MALDI-MS imaging with cellular resolution as well as an efficient generation of multiply charged MALDI ions by use of heated vacuum interfaces. This article reviews these recent innovations and gives the author's personal outlook of possible future developments.
基质辅助激光解吸/电离质谱（MALDI-MS）被广泛用于表征大型、热不稳定生物分子。这种分析技术的优点包括高灵敏度、稳健性、高通量能力以及适用于广泛的化合物类别。多年来，MALDI-MS也越来越多地用于质谱成像以及临床研究的其他领域。最近，提出了几个有可能进一步提升MALDI性能特征的新概念。这些创新包括具有低质子亲和力以实现分析物分子特别高效质子化的新型基质、使用波长可调谐激光器以实现最佳激发条件以及使用液体基质以改善定量。仪器改进还使得具有细胞分辨率的MALDI-MS成像以及通过使用加热真空接口高效生成多电荷MALDI离子成为可能。本文回顾了这些近期的创新，并给出了作者对未来可能发展的个人展望。
