Caldera Sandy Milena, Jaramillo María Cristina, Cochero Suljey, Pérez-Doria Alveiro, Bejarano Eduar Elías
Grupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia.
Secretaría de Salud de Sucre Dasssalud, Sincelejo, Colombia.
Biomedica. 2013 Sep;33 Suppl 1:89-98.
Aedes aegypti is the principal vector of dengue in urban areas. Despite its epidemiological importance, the genetic variability of Colombian populations of this species is unknown.
To determine the genetic variability of mitocondrial gene ND4, which codes for subunit 4 of the enzyme NADH deshydrogenase, between populations of Ae. aegypti from municipalities of Sincelejo and Guaranda. The incidences of dengue reported from these two localities are high and low, respectively.
Genetic material extracted from 36 females of Ae. aegypti was used to determine the partial sequence of the mitocondrial gene ND4 as well as to estimate the parameters of nucleotidic and haplotypic diversities, genetic structure and gene flow between the Sincelejo and Guaranda populations. The molecular variance was also analysed and a haplotypic network constructed.
In all 36 nucleotide sequences of 282 pb were obtained. These presented 12 polymorphic sites and could grouped into 10 haplotypes, two of them present in both populations, three exclusive to the Sincelejo population and five to that of Guaranda. The estimators of genetic structure (FST = 0.15) and gene flow (Nm = 1.40) are both indicative of genetic differentiation and a limited exchange of genes between the populations.
The Sincelejo and Guaranda populations of Ae. aegypti are genetically divergent.
埃及伊蚊是城市地区登革热的主要传播媒介。尽管其在流行病学上具有重要意义，但该物种在哥伦比亚种群中的遗传变异性尚不清楚。
确定来自辛塞莱霍和瓜兰达市的埃及伊蚊种群之间线粒体基因ND4的遗传变异性，该基因编码NADH脱氢酶的亚基4。这两个地区报告的登革热发病率分别为高和低。
从36只埃及伊蚊雌性个体中提取遗传物质，用于确定线粒体基因ND4的部分序列，以及估计辛塞莱霍和瓜兰达种群之间的核苷酸和单倍型多样性参数、遗传结构和基因流。还分析了分子方差并构建了单倍型网络。
共获得了36条282 pb的核苷酸序列。这些序列有12个多态性位点，可分为10个单倍型，其中两个在两个种群中都存在，三个是辛塞莱霍种群特有的，五个是瓜兰达种群特有的。遗传结构估计值（FST = 0.15）和基因流估计值（Nm = 1.40）均表明种群间存在遗传分化和有限的基因交换。
辛塞莱霍和瓜兰达的埃及伊蚊种群在遗传上存在差异。
