Kordower J H, Bartus R T, Marciano F F, Gash D M
Department of Neurobiology and Anatomy, University of Rochester School of Medicine, New York 14642.
J Comp Neurol. 1989 Jan 22;279(4):528-45. doi: 10.1002/cne.902790403.
While the cholinergic projection from the nucleus basalis to the cortical mantle has received considerable attention, a similar projection to the magnocellular basal nucleus of the amygdala has not been studied in such detail. The present study analyzed the cholinergic basal forebrain projection to the amygdala in the Cebus apella monkey by using combined tract-tracing and immunocytochemical techniques. As a foundation for this assessment, the morphological and cytoarchitectonic organization of the cholinergic telencephalic system of the New World C. apella monkey was examined by using choline acetyltransferase (ChAT) immunocytochemistry. Although there were minor differences, the telencephalic cholinergic system of Cebus monkeys is similar to that seen in Old World nonhuman primates. ChAT-immunoreactive neurons were observed throughout the Ch1-4 regions of the basal forebrain, with subdivisions of the Ch4 region similar to those previously described (Mesulam et al., '83a). Most cholinergic neurons were hyperchromic and magnocellular; however, some neurons were parvicellular. Like most species, cholinergic neurons were also observed throughout the striatum. However, unlike in rodents, cholinergic perikarya were not observed within the cortex or hippocampus. To analyze the cholinergic fiber projections from the basal forebrain to the amygdala, monkeys received an intraamygdaloid injection of the retrograde tracer horseradish peroxidase conjugated to wheat germ agglutinin. Retrogradely labeled neurons that colocalized ChAT or acetylcholinesterase (AChE) were found predominantly in the anterolateral portion of the CH4 region. Fewer double-labeled neurons were found in the anteromedial and intermediate portion of CH4 and in the CH3 region. Neurons that exhibited retrograde labeling were only occasionally discerned in the posterior portions of the CH4 region, in the medullary laminae of the globus pallidus, or lodged within the internal capsule. These data are discussed in terms of the putative role this cholinergic input might play in cognitive processing in primates.
虽然从基底核到皮质被盖的胆碱能投射受到了相当多的关注，但到杏仁核大细胞基底核的类似投射尚未得到如此详细的研究。本研究通过联合示踪和免疫细胞化学技术，分析了僧帽猴基底前脑向杏仁核的胆碱能投射。作为该评估的基础，使用胆碱乙酰转移酶（ChAT）免疫细胞化学检查了新大陆僧帽猴胆碱能端脑系统的形态和细胞构筑组织。尽管存在一些细微差异，但僧帽猴的端脑胆碱能系统与旧世界非人类灵长类动物的相似。在基底前脑的Ch1 - 4区域均观察到ChAT免疫反应性神经元，Ch4区域的细分与先前描述的相似（Mesulam等人，1983a）。大多数胆碱能神经元是深色的且为大细胞；然而，一些神经元是小细胞。与大多数物种一样，在整个纹状体内也观察到了胆碱能神经元。然而，与啮齿动物不同，在皮质或海马内未观察到胆碱能核周体。为了分析从基底前脑到杏仁核的胆碱能纤维投射，给猴子在杏仁核内注射了与小麦胚芽凝集素结合的逆行示踪剂辣根过氧化物酶。共定位ChAT或乙酰胆碱酯酶（AChE）的逆行标记神经元主要位于CH4区域的前外侧部分。在CH4的前内侧和中间部分以及CH3区域发现的双标记神经元较少。仅偶尔在CH4区域的后部、苍白球的髓板或内囊内发现有逆行标记的神经元。根据这种胆碱能输入在灵长类动物认知加工中可能发挥的假定作用对这些数据进行了讨论。
