Everitt B J, Sirkiä T E, Roberts A C, Jones G H, Robbins T W
Department of Anatomy, University of Cambridge, England.
J Comp Neurol. 1988 May 22;271(4):533-58. doi: 10.1002/cne.902710406.
The distribution of choline acetyltransferase-immunoreactive (ChAT-IR) neurons was studied in the brain of the common marmoset by using immunohistochemistry. ChAT-IR neurons were found in the medial septal nucleus, vertical and horizontal limb nuclei of the diagonal band, the nucleus basalis of Meynert, pedunculopontine nucleus and laterodorsal tegmental nucleus, and also in the striatum, habenula, and brainstem cranial nerve motor nuclei. The organization of ChAT-IR neurons in the basal forebrain, midbrain, and pons is consistent with the Ch1-Ch6 nomenclature introduced by Mesulam et al. ('83). The combination of the retrograde transport of HRP-WGA with ChAT immunohistochemistry revealed the distribution of neurons in the Ch4 cell group projecting to the dorsolateral prefrontal cortex. The activity of ChAT was highest in limbic cortical structures, such as the hippocampus, and lowest in association areas of the neocortex. Lesions at various loci in the basal forebrain resulted in differential patterns of ChAT loss in the cortex, which suggests some degree of topographical organization of Ch4 projections to the cortical mantle.
采用免疫组织化学方法，研究了普通狨猴脑中胆碱乙酰转移酶免疫反应阳性（ChAT-IR）神经元的分布。在内侧隔核、斜角带垂直和水平肢核、迈内特基底核、脚桥核和脑桥背外侧被盖核，以及纹状体、缰核和脑干脑神经运动核中均发现了ChAT-IR神经元。基底前脑、中脑和脑桥中ChAT-IR神经元的组织与Mesulam等人（1983年）提出的Ch1-Ch6命名法一致。HRP-WGA逆行运输与ChAT免疫组织化学相结合，揭示了投射到背外侧前额叶皮质的Ch4细胞群中神经元的分布。ChAT的活性在边缘皮质结构（如海马体）中最高，而在新皮质的联合区域中最低。基底前脑不同位点的损伤导致皮质中ChAT丧失的不同模式，这表明Ch4向皮质被膜的投射存在一定程度的拓扑组织。
