Chen Jiayu, Chen Jiaqi, Wang Xintai, Liu Chibo
Medical School of Taizhou University, Taizhou, Zhejiang 318000, China.
College of Life Science, Jilin University, Changchun, Jilin, 136000.
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):15-22. eCollection 2014.
It is well known that various polysaccharides present anti-tumour effects by inducing cell apoptosis and immunomodulation. However, it is still unclear about the roles of polysaccharides isolated from Artemisia apiacea (HQG) to hepatoma and its underlying mechanism. The objective of the study was to examine the anti-hepatoma effects of HQG and its related mechanism.
HQG was prepared in house and the quality and purity were confirmed by infra-red spectrum and gel permeation chromatography (GPC). Tumour-bearing mice induced by injection of mouse hepatoma H22 cells were used to evaluate the tumour growth inhibition by HQG administration. Cell immunostaining, JC1 staining and flow cytometer were performed to examine the cell apoptosis, mitochondrial membrane potential change and immunomodulation in response to HQG treatment.
HQG treatment inhibited hepatoma growth in tumour-bearing mice. Cell apoptosis rate of human hepatoma 7402 cells and of the cells from ascites in tumour-bearing mice was increased after HQG treatment. Mitochondrial membrane potential in human hepatoma 7402 cells was decreased after HQG treatment. CD4+ and CD8+ T-lymphocytes subpopulation was increased while the ratio of CD4+/ CD8+ decreased in tumour-bearing mice after HQG administration. IFN-γ and IL-4 secretion was increased in spleen lymphocytes in tumour-bearing mice after HQG administration.
The study concluded that polysaccharides isolated from Artemisia apiacea (HQG) can inhibit hepatoma cell growths by facilitating cell apoptosis and immuno-defence.
众所周知，多种多糖可通过诱导细胞凋亡和免疫调节发挥抗肿瘤作用。然而，从茵陈蒿中分离得到的多糖（HQG）对肝癌的作用及其潜在机制仍不清楚。本研究的目的是探讨HQG的抗肝癌作用及其相关机制。
在实验室制备HQG，并通过红外光谱和凝胶渗透色谱法（GPC）确认其质量和纯度。采用注射小鼠肝癌H22细胞诱导的荷瘤小鼠，评估给予HQG后对肿瘤生长的抑制作用。进行细胞免疫染色、JC1染色和流式细胞术，以检测HQG处理后细胞凋亡、线粒体膜电位变化和免疫调节情况。
HQG处理可抑制荷瘤小鼠肝癌生长。HQG处理后，人肝癌7402细胞及荷瘤小鼠腹水细胞的凋亡率增加。HQG处理后人肝癌7402细胞的线粒体膜电位降低。给予HQG后，荷瘤小鼠的CD4+和CD8+ T淋巴细胞亚群增加，而CD4+/CD8+比值降低。给予HQG后，荷瘤小鼠脾淋巴细胞中IFN-γ和IL-4分泌增加。
本研究得出结论，从茵陈蒿中分离得到的多糖（HQG）可通过促进细胞凋亡和免疫防御抑制肝癌细胞生长。
