龙葵多糖对荷瘤小鼠细胞免疫功能的影响研究
Study on the effect of polysaccharides from Solanum nigrum Linne on cellular immune function in tumour-bearing mice.
作者信息
Chen Hai, Qi Xiaodong
机构信息
Department of Galactophore, The Military General Hospital of Beijing PLA, Beijing, China.
出版信息
Afr J Tradit Complement Altern Med. 2013 May 16;10(4):41-6. doi: 10.4314/ajtcam.v10i4.7. eCollection 2013.
We investigated the anti-tumour effect of polysaccharides from Solanum nigrum Linne, and its relationship with the immune function of tumour-bearing organisms. MTT assay was used to observe the effect of different doses of polysaccharides from Solanum nigrum Linne on proliferation of lymphocytes in tumour-bearing mice. ELISA assay was also used to detect the levels of IL-2 in mice, and a laser scanning confocal microscope was used to detect the effect of polysaccharides from Solanum nigrum Linne on intralymphocytic free calcium ion concentration in tumour-bearing mice. Different doses of polysaccharides from Solanum nigrum Linne significantly inhibited the growth of mouse H22 solid tumours, improved the survival time of tumour-bearing mice, increased the proliferation of lymphocytes, elevated the levels of IL-2, and increased the concentration of calcium ions in the lymphocytes. Polysaccharides from Solanum nigrum Linne have certain anti-tumour effect, which is related with the cellular immune function that regulates the body.
我们研究了龙葵多糖的抗肿瘤作用及其与荷瘤机体免疫功能的关系。采用MTT法观察不同剂量龙葵多糖对荷瘤小鼠淋巴细胞增殖的影响。采用ELISA法检测小鼠IL-2水平，并用激光扫描共聚焦显微镜检测龙葵多糖对荷瘤小鼠淋巴细胞内游离钙离子浓度的影响。不同剂量的龙葵多糖能显著抑制小鼠H22实体瘤的生长，提高荷瘤小鼠的生存时间，增加淋巴细胞的增殖，提高IL-2水平，并增加淋巴细胞内钙离子浓度。龙葵多糖具有一定的抗肿瘤作用，这与调节机体的细胞免疫功能有关。
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