Kampf Günter, Degenhardt Stina, Lackner Sibylle, Ostermeyer Christiane
Bode Science Center, Bode Chemie GmbH, Hamburg, Germany ; Institut für Hygiene und Umweltmedizin, Ernst-Moritz-Arndt Universität Greifswald, Germany.
Microbiology, Bode Chemie GmbH, Hamburg, Germany.
GMS Hyg Infect Control. 2014 Mar 7;9(1):Doc09. doi: 10.3205/dgkh000229. eCollection 2014.
It has recently been reported that reusable dispensers for surface disinfection tissues may be contaminated, especially with adapted Achromobacter species 3, when products based on surface-active ingredients are used. Fresh solution may quickly become recontaminated if dispensers are not processed adequately.
We evaluated the abilities of six manual and three automatic processes for processing contaminated dispensers to prevent recolonisation of a freshly-prepared disinfectant solution (Mikrobac forte 0.5%). Dispensers were left at room temperature for 28 days. Samples of the disinfectant solution were taken every 7 days and assessed quantitatively for bacterial contamination.
All automatic procedures prevented recolonisation of the disinfectant solution when a temperature of 60-70°C was ensured for at least 5 min, with or without the addition of chemical cleaning agents. Manual procedures prevented recontamination of the disinfectant solution when rinsing with hot water or a thorough cleaning step was performed before treating all surfaces with an alcohol-based disinfectant or an oxygen-releaser. Other cleaning and disinfection procedures, including the use of an alcohol-based disinfectant, did not prevent recolonisation.
These results indicate that not all processes are effective for processing reusable dispensers for surface-disinfectant tissues, and that a high temperature during the cleaning step or use of a biofilm-active cleaning agent are essential.
最近有报道称，当使用基于表面活性成分的产品时，用于表面消毒纸巾的可重复使用分配器可能会被污染，尤其是被适应性产碱杆菌3型污染。如果分配器处理不当，新鲜溶液可能会很快再次被污染。
我们评估了六种手动和三种自动处理受污染分配器的方法，以防止新配制的消毒溶液（0.5%强力微生物消毒剂）再次被污染。将分配器在室温下放置28天。每7天采集一次消毒溶液样本，并对细菌污染进行定量评估。
当确保温度在60 - 70°C至少持续5分钟时，无论是否添加化学清洁剂，所有自动程序都能防止消毒溶液再次被污染。当在使用酒精基消毒剂或氧释放剂处理所有表面之前，先用热水冲洗或进行彻底清洁步骤时，手动程序可防止消毒溶液再次被污染。其他清洁和消毒程序，包括使用酒精基消毒剂，都不能防止再次被污染。
这些结果表明，并非所有处理方法对处理用于表面消毒纸巾的可重复使用分配器都有效，清洁步骤中的高温或使用生物膜活性清洁剂至关重要。
