Department of Cardiothorac Surgery, Affiliated People's Hospital of Jiangsu University , Zhenjiang , P.R. China and.
Biomarkers. 2014 May;19(3):252-8. doi: 10.3109/1354750X.2014.902997. Epub 2014 Mar 24.
The variant alleles of the functional polymorphisms, VEGF -2578 C > A, -1498 T > C and +936 C > T, were not associated with risk of esophageal cancer and lymph node metastasis. Compared with the most common haplotype, C-2578T-1498C+936, the A-2578C-1498C+936 haplotype was associated with a borderline significantly increased risk of esophageal cancer. C-2578C-1498C+936 and A-2578T-1498T+936 haplotypes were associated with significantly increased risk of esophageal cancer. Vascular endothelial growth factor (VEGF) is a potent stimulator for angiogenesis. It has been implicated in the growth and metastasis of esophageal cancer. Three functional single nucleotide polymorphisms (SNPs) of VEGF (-2578 C > A, -1498 T > C and +936 C > T) are known to be related to VEGF expression. We conducted a case-control study to evaluate the effects of these three functional SNPs on the development of esophageal cancer and lymph node metastasis. A total of 497 cases and 380 controls were analyzed. Genotypes were determined by matrix assisted laser desorption/ionization time-of-flight mass spectrometry and direct sequence methods. The variant alleles of the functional polymorphisms VEGF -2578 C > A, -1498 T > C and +936 C > T SNPs were not associated with esophageal cancer risk. These VEGF genotypes were not associated with the risk of esophageal cancer after stratification. Furthermore, no association was observed between VEGF -2578 C > A, -1498 T > C and +936 C > T polymorphisms and lymph node metastasis. Compared with the most common haplotype C-2578T-1498C+936, the A-2578C-1498C+936 haplotype was associated with a borderline significantly increased risk of esophageal cancer. C-2578C-1498C+936 and A-2578T-1498T+936 haplotypes were associated with significantly increased risk of esophageal cancer. Variants in the functional polymorphisms of VEGF may not contribute to esophageal cancer and lymph node metastasis susceptibility. VEGF A-2578C-1498C+936, C-2578C-1498C+936 and A-2578T-1498T+936 haplotypes may be associated with increased risk of esophageal cancer. However, our results were obtained with a limited sample size and therefore this is a preliminary conclusion. Validation by a larger study with more diverse ethnic populations is needed.
该功能多态性的变异等位基因，VEGF-2578 C> A，-1498 T> C 和+936 C> T，与食管癌和淋巴结转移的风险无关。与最常见的单倍型相比，A-2578C-1498C+936 单倍型与食管癌的风险呈临界显著增加相关。C-2578C-1498C+936 和 A-2578T-1498T+936 单倍型与食管癌的风险显著增加相关。血管内皮生长因子（VEGF）是血管生成的有力刺激物。它与食管癌的生长和转移有关。已知 VEGF 的三个功能单核苷酸多态性（SNP）（-2578 C> A、-1498 T> C 和+936 C> T）与 VEGF 表达有关。我们进行了一项病例对照研究，以评估这三个功能 SNP 对食管癌发展和淋巴结转移的影响。分析了 497 例病例和 380 例对照。通过基质辅助激光解吸/电离飞行时间质谱和直接测序方法确定基因型。功能多态性 VEGF-2578 C> A、-1498 T> C 和+936 C> T SNP 的变异等位基因与食管癌风险无关。对这些 VEGF 基因型进行分层后，与食管癌风险无关。此外，VEGF-2578 C> A、-1498 T> C 和+936 C> T 多态性与淋巴结转移无关。与最常见的单倍型 C-2578T-1498C+936 相比，A-2578C-1498C+936 单倍型与食管癌的风险呈临界显著增加相关。C-2578C-1498C+936 和 A-2578T-1498T+936 单倍型与食管癌的风险显著增加相关。VEGF 功能多态性的变异可能不会导致食管癌和淋巴结转移的易感性。VEGF A-2578C-1498C+936、C-2578C-1498C+936 和 A-2578T-1498T+936 单倍型可能与食管癌风险增加相关。然而，我们的结果是基于有限的样本量得出的，因此这只是一个初步结论。需要通过更大的具有更多不同种族人群的研究来验证。
