Londei P, Altamura S, Sanz J L, Amils R
Dipartimento di Biopatologia Umana, Università di Roma, Policlinico Umberto I, Italy.
Mol Gen Genet. 1988 Sep;214(1):48-54. doi: 10.1007/BF00340178.
The effect of selected aminoglycoside antibiotics on the translational accuracy of poly(U) programmed ribosomes derived from the thermophilic archaebacteria Thermoplasma acidophilum, Sulfolobus solfataricus, Thermococcus celer and Desulfurococcus mobilis has been determined. Under optimum temperature and ionic conditions for polyphenylalanine synthesis, the four species investigated are found to be markedly diverse in their response to the miscoding-inducing action of aminoglycoside antibiotics. T. acidophilum is sensitive to all of the compounds tested except streptomycin; S. solfataricus responds to paromomycin and to hygromycin B; T. celer is only affected by neomycin, and D. mobilis is refractory to all drugs. The only feature shared by the four species under study, and by all archaebacteria so far investigated, is their complete insensitivity to streptomycin. The structural and phylogenetic implications of the remarkable diversity encountered among archaebacterial ribosomes in their susceptibility to aminoglycosides are discussed.
已测定了所选氨基糖苷类抗生素对源自嗜热古细菌嗜酸热原体、嗜热栖热菌、嗜热栖热球菌和运动脱硫球菌的聚（U）编程核糖体翻译准确性的影响。在聚苯丙氨酸合成的最佳温度和离子条件下，发现所研究的这四个物种对氨基糖苷类抗生素的误编码诱导作用的反应明显不同。嗜酸热原体对除链霉素外的所有测试化合物敏感；嗜热栖热菌对巴龙霉素和潮霉素B有反应；嗜热栖热球菌仅受新霉素影响，而运动脱硫球菌对所有药物均无反应。所研究的这四个物种以及迄今为止研究的所有古细菌共有的唯一特征是它们对链霉素完全不敏感。讨论了古细菌核糖体对氨基糖苷类药物敏感性方面显著差异的结构和系统发育意义。
