大肠杆菌16S和23S核糖体RNA基因中的抗生素抗性突变
Antibiotic resistance mutations in 16S and 23S ribosomal RNA genes of Escherichia coli.
作者信息
Sigmund C D, Ettayebi M, Morgan E A
出版信息
Nucleic Acids Res. 1984 Jun 11;12(11):4653-63. doi: 10.1093/nar/12.11.4653.
Abstract
Recombinant DNA and classic genetic procedures were used to map a spectinomycin resistance mutation to a 121 base pair region of a 16S RNA gene and a macrolide-lincosamide-streptogramin type B resistance mutation to a 32 base pair region of a 23S RNA gene. DNA sequence analysis of these regions revealed that spectinomycin resistance results from a C/G to T/A transition at position 1192 of a 16S RNA gene. Resistance to macrolide, lincosamide and streptogramin type B antibiotics results from an A/T to T/A transversion at position 2058 of a 23S RNA gene. The alteration in 16S RNA is in a sequence that can participate in alternate base pairing arrangements that have been proposed to be involved in the translocation process. The alteration in 23S RNA identifies sequences important to peptidyl transfer.
摘要
利用重组DNA和经典遗传学方法，将壮观霉素抗性突变定位到16S RNA基因的一个121碱基对区域，将大环内酯-林可酰胺-链阳菌素B型抗性突变定位到23S RNA基因的一个32碱基对区域。对这些区域的DNA序列分析表明，壮观霉素抗性是由16S RNA基因第1192位的C/G到T/A转换引起的。对大环内酯、林可酰胺和链阳菌素B型抗生素的抗性是由23S RNA基因第2058位的A/T到T/A颠换引起的。16S RNA中的改变发生在一个可参与替代碱基配对排列的序列中，这些排列被认为与转位过程有关。23S RNA中的改变确定了对肽基转移很重要的序列。
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