雷公藤红素脂质体对前列腺癌细胞的抗癌活性评估。
Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells.
作者信息
Wolfram Joy, Suri Krishna, Huang Yi, Molinaro Roberto, Borsoi Carlotta, Scott Bronwyn, Boom Kathryn, Paolino Donatella, Fresta Massimo, Wang Jianghua, Ferrari Mauro, Celia Christian, Shen Haifa
机构信息
Department of Nanomedicine, Houston Methodist Research Institute , Houston, TX , USA .
出版信息
J Microencapsul. 2014;31(5):501-7. doi: 10.3109/02652048.2013.879932. Epub 2014 Mar 24.
Abstract
CONTEXT
Celastrol, a natural compound derived from the herb Tripterygium wilfordii, is known to have anticancer activity, but is not soluble in water.
OBJECTIVE
Formation of celastrol liposomes, to avoid the use of toxic solubilising agents.
MATERIALS AND METHODS
Two different formulations of PEGylated celastrol liposomes were fabricated. Liposomal characteristics and serum stability were determined using dynamic light scattering. Drug entrapment efficacy and drug release were measured spectrophotometrically. Cellular internalisation and anticancer activity was measured in prostate cancer cells.
RESULTS
Liposomal celastrol displayed efficient serum stability, cellular internalisation and anticancer activity, comparable to that of the free drug reconstituted in dimethyl sulfoxide.
DISCUSSION AND CONCLUSION
Liposomal celastrol can decrease the viability of prostate cancer cells, while eliminating the need for toxic solubilising agents.
摘要
背景
雷公藤红素是一种从草药雷公藤中提取的天然化合物，已知具有抗癌活性，但不溶于水。
目的
制备雷公藤红素脂质体，以避免使用有毒增溶剂。
材料与方法
制备了两种不同配方的聚乙二醇化雷公藤红素脂质体。使用动态光散射测定脂质体特性和血清稳定性。采用分光光度法测定药物包封率和药物释放。在前列腺癌细胞中测定细胞内化和抗癌活性。
结果
脂质体雷公藤红素显示出高效的血清稳定性、细胞内化和抗癌活性，与在二甲基亚砜中重构的游离药物相当。
讨论与结论
脂质体雷公藤红素可降低前列腺癌细胞的活力，同时无需使用有毒增溶剂。
相似文献
1
Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells.
J Microencapsul. 2014;31(5):501-7. doi: 10.3109/02652048.2013.879932. Epub 2014 Mar 24.
2
Celastrol, a triterpene extracted from the Chinese "Thunder of God Vine," is a potent proteasome inhibitor and suppresses human prostate cancer growth in nude mice.
Cancer Res. 2006 May 1;66(9):4758-65. doi: 10.1158/0008-5472.CAN-05-4529.
3
Celastrol isolated from Tripterygium regelii induces apoptosis through both caspase-dependent and -independent pathways in human breast cancer cells.
Food Chem Toxicol. 2011 Feb;49(2):527-32. doi: 10.1016/j.fct.2010.11.044. Epub 2010 Dec 4.
4
Sugar-decorated mesoporous silica nanoparticles as delivery vehicles for the poorly soluble drug celastrol enables targeted induction of apoptosis in cancer cells.
Eur J Pharm Biopharm. 2015 Oct;96:11-21. doi: 10.1016/j.ejpb.2015.07.009. Epub 2015 Jul 14.
5
Downregulation of miR-17-92a cluster promotes autophagy induction in response to celastrol treatment in prostate cancer cells.
Biochem Biophys Res Commun. 2016 Sep 16;478(2):804-10. doi: 10.1016/j.bbrc.2016.08.029. Epub 2016 Aug 5.
6
Celastrol, an active constituent of the TCM plant Tripterygium wilfordii Hook.f., inhibits prostate cancer bone metastasis.
Prostate Cancer Prostatic Dis. 2017 Jun;20(2):156-164. doi: 10.1038/pcan.2016.61. Epub 2017 Feb 14.
7
Preparation, characterization, and assessment of the antiglioma effects of liposomal celastrol.
Anticancer Drugs. 2012 Jun;23(5):515-24. doi: 10.1097/CAD.0b013e3283514b68.
8
Effects of Celastrol on growth inhibition of U937 leukemia cells through the regulation of the Notch1/NF-kappaB signaling pathway in vitro.
Chin J Cancer. 2010 Apr;29(4):385-90. doi: 10.5732/cjc.009.10526.
9
Nanoencapsulation of natural triterpenoid celastrol for prostate cancer treatment.
Int J Nanomedicine. 2015 Oct 30;10:6835-46. doi: 10.2147/IJN.S93752. eCollection 2015.
10
Celastrol induced DNA damage, cell cycle arrest, and apoptosis in human rheumatoid fibroblast-like synovial cells.
Am J Chin Med. 2013;41(3):615-28. doi: 10.1142/S0192415X13500432.
引用本文的文献
1
Liposomal Encapsulation of Carob ( L.) Pulp Extract: Design, Characterization, and Controlled Release Assessment.
Pharmaceutics. 2025 Jun 13;17(6):776. doi: 10.3390/pharmaceutics17060776.
2
Formulation and In-Vitro Testing of Nebulized Camostat Mesylate Loaded Nanoliposomes for the Treatment of SARS-CoV- 2 Infection.
AAPS PharmSciTech. 2025 May 16;26(5):139. doi: 10.1208/s12249-025-03099-3.
3
Nanocarriers for Delivery of Anticancer Drugs: Current Developments, Challenges, and Perspectives.
Pharmaceutics. 2024 Nov 27;16(12):1527. doi: 10.3390/pharmaceutics16121527.
4
Clinical insights into nanomedicine and biosafety: advanced therapeutic approaches for common urological cancers.
Front Oncol. 2024 Aug 13;14:1438297. doi: 10.3389/fonc.2024.1438297. eCollection 2024.
5
Tripterin liposome relieves severe acute respiratory syndrome as a potent COVID-19 treatment.
Signal Transduct Target Ther. 2022 Dec 24;7(1):399. doi: 10.1038/s41392-022-01283-6.
6
Sequential Delivery of Novel Triple Drug Combination via Crosslinked Alginate/Lactoferrin Nanohybrids for Enhanced Breast Cancer Treatment.
Pharmaceutics. 2022 Nov 8;14(11):2404. doi: 10.3390/pharmaceutics14112404.
7
Vascular Implications of COVID-19: Role of Radiological Imaging, Artificial Intelligence, and Tissue Characterization: A Special Report.
J Cardiovasc Dev Dis. 2022 Aug 15;9(8):268. doi: 10.3390/jcdd9080268.
8
Celastrol: A lead compound that inhibits SARS-CoV-2 replication, the activity of viral and human cysteine proteases, and virus-induced IL-6 secretion.
Drug Dev Res. 2022 Nov;83(7):1623-1640. doi: 10.1002/ddr.21982. Epub 2022 Aug 21.
9
Deep Learning Paradigm for Cardiovascular Disease/Stroke Risk Stratification in Parkinson's Disease Affected by COVID-19: A Narrative Review.
Diagnostics (Basel). 2022 Jun 24;12(7):1543. doi: 10.3390/diagnostics12071543.
10
Methotrexate-Lactoferrin Targeted Exemestane Cubosomes for Synergistic Breast Cancer Therapy.
Front Chem. 2022 Mar 22;10:847573. doi: 10.3389/fchem.2022.847573. eCollection 2022.
本文引用的文献
1
Liposomal chemotherapeutics.
Future Oncol. 2013 Dec;9(12):1849-59. doi: 10.2217/fon.13.146.
2
Shrinkage of pegylated and non-pegylated liposomes in serum.
Colloids Surf B Biointerfaces. 2014 Feb 1;114:294-300. doi: 10.1016/j.colsurfb.2013.10.009. Epub 2013 Oct 24.
3
Ex-vivo/in-vitro anti-polyethylene glycol (PEG) immunoglobulin M production from murine splenic B cells stimulated by PEGylated liposome.
Biol Pharm Bull. 2013;36(11):1842-8. doi: 10.1248/bpb.b13-00562.
4
Anticancer activity of liposomal bergamot essential oil (BEO) on human neuroblastoma cells.
Colloids Surf B Biointerfaces. 2013 Dec 1;112:548-53. doi: 10.1016/j.colsurfb.2013.09.017. Epub 2013 Sep 17.
5
Polyethylenimine and chitosan carriers for the delivery of RNA interference effectors.
Expert Opin Drug Deliv. 2013 Dec;10(12):1653-68. doi: 10.1517/17425247.2013.840286. Epub 2013 Oct 4.
6
The accelerated blood clearance (ABC) phenomenon: clinical challenge and approaches to manage.
J Control Release. 2013 Nov 28;172(1):38-47. doi: 10.1016/j.jconrel.2013.07.026. Epub 2013 Aug 7.
7
Celastrol suppresses tumor cell growth through targeting an AR-ERG-NF-κB pathway in TMPRSS2/ERG fusion gene expressing prostate cancer.
PLoS One. 2013;8(3):e58391. doi: 10.1371/journal.pone.0058391. Epub 2013 Mar 6.
8
Anti-PEG IgM production via a PEGylated nano-carrier system for nucleic acid delivery.
Methods Mol Biol. 2013;948:35-47. doi: 10.1007/978-1-62703-140-0_4.
9
Liposomal paclitaxel formulations.
J Control Release. 2012 Nov 10;163(3):322-34. doi: 10.1016/j.jconrel.2012.09.006. Epub 2012 Sep 15.
10
Preparation, characterization, and assessment of the antiglioma effects of liposomal celastrol.
Anticancer Drugs. 2012 Jun;23(5):515-24. doi: 10.1097/CAD.0b013e3283514b68.
Zotero 插件