Shiromani P J, Armstrong D M, Gillin J C
San Diego Veterans Administration Medical Center, La Jolla, CA 92161.
Neurosci Lett. 1988 Dec 19;95(1-3):19-23. doi: 10.1016/0304-3940(88)90625-8.
In this study we determined that cholinergic neurons from the lateral dorsal tegmental (LDT) and peribrachial pontine region (PPG) innervate the medial pontine reticular formation (medial PRF), a region involved in the generation of REM sleep. Wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) was injected into the medial PRF and the brainstem tissue was processed using a combined retrograde transport/immunocytochemical procedure. Results showed that 10-15% of choline acetyltransferase (ChAT) immunoreactive neurons in the LDT and PPG project to the medial PRF. It is hypothesized that these neurons play an important role in the generation of the REM sleep state.
在本研究中，我们确定来自外侧背盖区（LDT）和臂旁脑桥区域（PPG）的胆碱能神经元支配脑桥内侧网状结构（内侧PRF），该区域参与快速眼动睡眠的产生。将小麦胚凝集素结合辣根过氧化物酶（WGA-HRP）注入内侧PRF，并使用逆行运输/免疫细胞化学联合程序处理脑干组织。结果显示，LDT和PPG中10%-15%的胆碱乙酰转移酶（ChAT）免疫反应性神经元投射到内侧PRF。据推测，这些神经元在快速眼动睡眠状态的产生中起重要作用。
