Van Daele Jeroen, Blancquaert Dieter, Kiekens Filip, Van Der Straeten Dominique, Lambert Willy E, Stove Christophe P
Laboratory of Toxicology, Faculty of Pharmaceutical Sciences, Ghent University , Harelbekestraat 72, Gent 9000, Belgium.
Laboratory of Functional Plant Biology, Department of Physiology, Ghent University , K.L. Ledeganckstraat 35, Gent 9000, Belgium.
J Agric Food Chem. 2014 Apr 9;62(14):3092-3100. doi: 10.1021/jf500753v. Epub 2014 Mar 31.
An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the profiling of six folate species in potatoes. The calibration curves cover a wide, linear range (the lower and upper limits of quantitation range between 0.22-0.24 and 216.07-242.28 μg/100 g of fresh weight), allowing sensitive determination in small amounts of potato flesh. With a single exception, the acceptance criteria for intra- and interday precision and accuracy were met: for all quality controls, the percent relative standard deviation and the percent bias were lower than 15% (or 20% at the lower limit of quantitation). Application of the method on tubers at different stages of maturation demonstrated the large variability within a single variety: the folate content and polyglutamylation rate varied between 10.35 and 24.01 μg/100 g of fresh weight and between 4.96% and 60.49%, respectively. Additionally, the two-dimensional folate profiling of mature tubers demonstrated an increase in folate from center to peel, combined with a stable species distribution and polyglutamylation rate.
建立了一种超高效液相色谱-串联质谱法，并对其进行了验证，用于分析马铃薯中六种叶酸种类。校准曲线涵盖了较宽的线性范围（定量范围的下限和上限分别为0.22 - 0.24和216.07 - 242.28μg/100g鲜重），能够灵敏地测定少量马铃薯果肉中的叶酸。除了一个例外，日内和日间精密度与准确度的验收标准均得到满足：对于所有质量控制样品，相对标准偏差百分比和偏差百分比均低于15%（或在定量下限处为20%）。该方法应用于不同成熟阶段的块茎，结果表明同一品种内存在很大差异：叶酸含量和多聚谷氨酸化率分别在10.35至24.01μg/100g鲜重之间以及4.96%至60.49%之间变化。此外，成熟块茎的二维叶酸谱分析表明，从块茎中心到表皮叶酸含量增加，同时叶酸种类分布和多聚谷氨酸化率保持稳定。
