采用高效液相色谱-串联质谱法对薯片中溴酸盐的痕量分析。

Trace analysis of bromate in potato snacks using high-performance liquid chromatography-tandem mass spectrometry.

机构信息

The Procter and Gamble Company, Global Analytical Capability, Mason, OH 45040, USA.

出版信息

J Agric Food Chem. 2010 Jul 28;58(14):8134-8. doi: 10.1021/jf902732e.

PMID:20583807

Abstract

A simple, sensitive, and selective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method in the negative-ion electrospray ionization (ESI(-)) mode was validated for the quantitation of bromate (BrO(3)(-)) in potato snacks. Ground snack specimens ( approximately 0.5 g/sample) are spiked with Br(18)O(3)(-), stable-isotope labeled bromate internal standard (IS), and vortexed with a mixture of distilled/deionized water (dd water) and heptane. Subsequently, the specimens are centrifuged, and a small portion of the aqueous extract is isolated, diluted with dd water (1:4), and analyzed by HPLC-MS/MS. The methodology has a quantitation range of 10-1000 ppb, an accuracy of 1.5-7.5%, and a precision of 5.2-13.4% across the concentration range.

摘要

建立了一种简单、灵敏、选择性好的高效液相色谱-串联质谱（HPLC-MS/MS）方法，用于检测土豆零食中的溴酸盐（BrO3-）。将粉碎的零食样品（约 0.5 g/样品）用 Br18O3-、稳定同位素标记的溴酸盐内标（IS）进行处理，用蒸馏水/去离子水（dd 水）和正庚烷的混合物涡旋，然后离心，取出一小部分水提取物，用 dd 水（1:4）稀释，用 HPLC-MS/MS 进行分析。该方法的定量范围为 10-1000 ppb，在整个浓度范围内的准确度为 1.5-7.5%，精密度为 5.2-13.4%。