Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
J Appl Microbiol. 2014 Jul;117(1):249-57. doi: 10.1111/jam.12503. Epub 2014 Apr 8.
To elucidate the Campylobacter jejuni population in organically farmed laying hens in Finland, multilocus sequence typing (MLST) was combined with characterization of clustered regularly interspaced short palindromic repeat (CRISPR) sequences.
A total of 147 Camp. jejuni isolates, collected from organically farmed laying hens from 18 farms in 2003-2004, were previously analysed by pulsed-field gel electrophoresis. In the present study, subsets of the isolates were further analysed by MLST and CRISPR sequences. Fourteen STs were found by MLST. ST-50 (27%, 7/18 farms), ST-3272 (20%, 8/18 farms), ST-45 (12%, 7/18 farms) and ST-356 (12%, 5/18 farms) were the most common STs. CRISPR types were identical among all isolates of ST-50 (ST-21 clonal complex (CC)) and the most variable among ST-45 (ST-45 CC).
ST-3272 (UA), a common ST in this study, has been infrequently detected in other hosts. Other major STs (ST-50 and ST-45) have been common in several hosts such as conventional poultry and bovines. CRISPR typing provided additional discrimination between isolates of certain dominant STs and could be useful in further epidemiological studies.
This study gives new information about MLST and CRISPR types of Camp. jejuni among organically farmed laying hens.
阐明芬兰有机养殖蛋鸡中的空肠弯曲杆菌种群，采用多位点序列分型（MLST）与聚类规则间隔短回文重复（CRISPR）序列特征相结合的方法。
2003-2004 年，从 18 个农场的有机养殖蛋鸡中采集了 147 株空肠弯曲杆菌分离株，此前曾用脉冲场凝胶电泳进行分析。在本研究中，进一步用 MLST 和 CRISPR 序列分析了分离株的子集。通过 MLST 发现了 14 种 ST。ST-50（27%，18 个农场中的 7 个）、ST-3272（20%，18 个农场中的 8 个）、ST-45（12%，18 个农场中的 7 个）和 ST-356（12%，18 个农场中的 5 个）是最常见的 ST。ST-50（ST-21 克隆复合体（CC））的所有分离株的 CRISPR 类型相同，而 ST-45 的最可变（ST-45 CC）。
本研究中常见的 ST-3272（UA）在其他宿主中很少检测到。其他主要 ST（ST-50 和 ST-45）在常规家禽和牛等多种宿主中很常见。CRISPR 分型为某些优势 ST 分离株提供了额外的鉴别能力，可用于进一步的流行病学研究。
本研究提供了关于有机养殖蛋鸡中空肠弯曲杆菌 MLST 和 CRISPR 类型的新信息。
