Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
J Genet Genomics. 2014 Mar 20;41(3):153-64. doi: 10.1016/j.jgg.2013.11.006. Epub 2013 Dec 13.
α-kleisins are core components of meiotic and mitotic cohesin complexes. Arabidopsis contains genes encoding four α-kleisins. SYN1, a REC8 ortholog, is essential for meiosis, while SYN2 and SYN4 appear to be SCC1 orthologs and function in mitosis. SYN3 is enriched in the nucleolus of meiotic and mitotic cells and is essential for megagametogenesis. It was recently shown that expression of SYN3-RNAi constructs in buds cause changes in meiotic gene expression that result in meiotic alterations. In this report we show that expression of SYN3 from the 35S promoter with either a c-terminal Myc or FAST tag causes a reduction in SYN1 mRNA levels that results in alterations in sister chromatid cohesion, homologous chromosome synapsis and synaptonemal complex formation during both male and female meiosis.
α- kleisins 是减数分裂和有丝分裂黏合复合物的核心组成部分。拟南芥含有编码四个α- kleisins 的基因。SYN1，一个 REC8 的同源物，对于减数分裂是必需的，而 SYN2 和 SYN4 似乎是 SCC1 的同源物，在有丝分裂中起作用。SYN3 在减数分裂和有丝分裂细胞的核仁中富集，并且对于大配子发生是必需的。最近的研究表明，在芽中表达 SYN3-RNAi 构建体导致减数分裂基因表达的变化，从而导致减数分裂的改变。在本报告中，我们显示，35S 启动子表达带或不带 C 端 Myc 或 FAST 标签的 SYN3，导致 SYN1 mRNA 水平降低，从而导致姐妹染色单体黏合、同源染色体联会和联会复合体形成在雄性和雌性减数分裂中均发生改变。
