Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy.
Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:107-18. doi: 10.1016/j.msec.2014.01.045. Epub 2014 Feb 6.
A glass belonging to the system SiO2-P2O5-CaO-MgO-Na2O-K2O was modified by introducing two different amounts of manganese oxide (MnO). Mn-doped glasses were prepared by melt and quenching technique and characterized by means of X-ray diffraction (XRD), scanning electron microscopy (SEM) observation and energy dispersion spectrometry (EDS) analysis. In vitro bioactivity test in simulated body fluid (SBF) showed a slight decrease in the reactivity kinetics of Mn-doped glasses compared to the glass used as control; however the glasses maintained a good degree of bioactivity. Mn-leaching test in SBF and minimum essential medium (MEM) revealed fluctuating trends probably due to a re-precipitation of Mn compounds during the bioactivity process. Cellular tests showed that all the Mn-doped glasses, up to a concentration of 50 μg/cm(2) (μg of glass powders/cm(2) of cell monolayer), did not produce cytotoxic effects on human MG-63 osteoblasts cultured for up to 5 days. Finally, biocompatibility tests demonstrated a good osteoblast proliferation and spreading on Mn-doped glasses and most of all that the Mn-doping can promote the expression of alkaline phosphatase (ALP) and some bone morphogenetic proteins (BMPs).
SiO2-P2O5-CaO-MgO-Na2O-K2O 系统的玻璃通过引入两种不同量的氧化锰（MnO）进行改性。采用熔融淬火技术制备 Mn 掺杂玻璃，并通过 X 射线衍射（XRD）、扫描电子显微镜（SEM）观察和能谱分析（EDS）进行表征。在模拟体液（SBF）中的体外生物活性测试表明，与用作对照的玻璃相比，Mn 掺杂玻璃的反应动力学略有降低;然而，玻璃保持了良好的生物活性。在 SBF 和最低必需培养基（MEM）中的 Mn 浸出试验显示出波动的趋势，这可能是由于在生物活性过程中 Mn 化合物的再沉淀所致。细胞试验表明，所有 Mn 掺杂玻璃，高达 50μg/cm(2)（玻璃粉末μg/cm(2)单层细胞）的浓度，对培养长达 5 天的人 MG-63 成骨细胞均无细胞毒性作用。最后，生物相容性试验表明 Mn 掺杂玻璃上具有良好的成骨细胞增殖和扩展，而且 Mn 掺杂可以促进碱性磷酸酶（ALP）和一些骨形态发生蛋白（BMPs）的表达。
