Yamaguchi H, Hirai S, Morimatsu M, Shoji M, Harigaya Y
College of Medical Care and Technology, Gunma University, Japan.
Acta Neuropathol. 1988;77(2):113-9. doi: 10.1007/BF00687420.
We studied the nature of diffuse type of senile plaques (SP) in the brains of six autopsied subjects with Alzheimer-type dementia (ATD). The densities of SP in the entorhinal cortex were evaluated using serial sections stained by four different methods. Compared with beta protein immunostaining (100% as a reference), the modified Bielschowsky stain (103%) and the periodic acid-methenamine silver (PAM) stain (109%) labeled similar numbers of SP, whereas the Bodian stain labeled only a minor proportion (42%) of these. The vast majority of Bodian-negative plaques were diffuse plaques, which were seen as ill-defined areas of fine fibrillar material after beta protein immunostain with formic acid pretreatment, modified Bielschowsky stain, and PAM stain. They were not stained by Congo red or periodic acid-Schiff stains. Double staining using Bodian and beta protein methods demonstrated that diffuse plaques were free of swollen neurites. Argyrophilia of the diffuse plaques shown by the modified Bielschowsky and PAM stains, became undetectable when sections were pretreated with formic acid. Such treatment made the diffuse plaques immunoreactive to beta protein antiserum, suggesting that diffuse plaques consisted mainly of amyloid, but not neuritic components. The diffuse plaques were distributed in various cortical areas and in the amygdala, and comprised a considerable population of the SP in the ATD brains.
我们研究了6例阿尔茨海默病型痴呆（ATD）尸检对象大脑中弥漫型老年斑（SP）的性质。使用四种不同方法染色的连续切片评估内嗅皮质中SP的密度。与β蛋白免疫染色（以100%作为参照）相比，改良 Bielschowsky 染色（103%）和高碘酸-亚甲胺银（PAM）染色（109%）标记的SP数量相似，而博迪安染色仅标记了其中一小部分（42%）。绝大多数博迪安染色阴性的斑块是弥漫性斑块，在用甲酸预处理后的β蛋白免疫染色、改良 Bielschowsky 染色和PAM染色后，这些斑块表现为细纤维物质的边界不清区域。它们不能被刚果红或过碘酸希夫染色。使用博迪安染色和β蛋白方法的双重染色表明，弥漫性斑块没有肿胀的神经突。经甲酸预处理后，改良 Bielschowsky 染色和PAM染色显示的弥漫性斑块的嗜银性消失。这种处理使弥漫性斑块对β蛋白抗血清具有免疫反应性，表明弥漫性斑块主要由淀粉样蛋白组成，而非神经炎性成分。弥漫性斑块分布于各个皮质区域和杏仁核，在ATD大脑的SP中占相当比例。
