Ikeda K, Haga C, Kosaka K, Oyanagi S
Tokyo Metropolitan Matsuzawa Hospital, Japan.
Acta Neuropathol. 1989;78(2):137-42. doi: 10.1007/BF00688201.
Numerous diffuse senile plaque-like structures (SPLSs) were found in the cerebral cortex from cases with dementia of the Alzheimer type by means of the methenamine-Bodian method. SPLSs varied in shape and size. They were never recognized in the original Bodian, PAS and Congo red preparations, but were positive with anti-beta-protein immunostaining and periodic-acid methenamine silver (PAM) methods, which are thought to specifically stain amyloid substance. With PAM electron microscopy, we found sparse aggregations of amorphous, often ramified, structures with fine granular silver deposits in SPLS. Routine electron microscopic examination on the same portion where SPLS were confirmed by PAM electron microscopy revealed amorphous, partially fibrous structures. These structures might be amyloid or amyloid-precursor substance. In SPLSs only a few degenerated neurites and astrocytic processes with glycogen granules were seen. We consider SPLSs to be a kind of senile plaque.
采用六亚甲基四胺 - 博迪安法，在阿尔茨海默型痴呆病例的大脑皮质中发现了大量弥漫性老年斑样结构（SPLSs）。SPLSs的形状和大小各不相同。在原始的博迪安、过碘酸希夫（PAS）和刚果红染色制剂中从未发现过它们，但用抗β蛋白免疫染色和高碘酸六亚甲基四胺银（PAM）法呈阳性，这两种方法被认为能特异性地染色淀粉样物质。通过PAM电子显微镜检查，我们发现在SPLSs中有稀疏的无定形、常呈分支状的结构聚集，伴有细小的颗粒状银沉积。在通过PAM电子显微镜确认有SPLSs的同一部位进行常规电子显微镜检查，发现有无定形的、部分呈纤维状的结构。这些结构可能是淀粉样蛋白或淀粉样前体物质。在SPLSs中，仅可见少数退变的神经突和含有糖原颗粒的星形胶质细胞突起。我们认为SPLSs是一种老年斑。
