Mercati Francesca, Pascucci Luisa, Curina Giovanni, Scocco Paola, Tardella Federico Maria, Dall'aglio Cecilia, Marini Carla, Ceccarelli Piero
Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy.
Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy.
Vet J. 2014 May;200(2):339-42. doi: 10.1016/j.tvjl.2014.02.018. Epub 2014 Feb 27.
The transplantation of multipotent mesenchymal stromal cells (MSCs) is a potentially promising therapy for the treatment of tendon and ligament injuries and some forms of articular pathology in horses. This study investigated the effects of storage conditions on MSCs. Equine adipose tissue-derived MSCs (eAd-MSCs) were stored at 4 °C and at room temperature (RT) for 24 and 48 h, and viability, doubling time, expression of CD44 and CD90 antigens, clonogenic/differentiation potentials, and karyotype were subsequently evaluated. The eAd-MSC viability was significantly affected by the storage conditions, while doubling time was not significantly altered. The findings indicate (1) that storage at 4 °C is preferable to RT as this results in greater numbers of viable, morphologically normal cells, and (2) that cells should be used within 24 h.
多能间充质基质细胞（MSCs）移植是治疗马肌腱和韧带损伤以及某些关节疾病的一种潜在的有前景的疗法。本研究调查了储存条件对MSCs的影响。将马脂肪组织来源的MSCs（eAd-MSCs）在4°C和室温（RT）下储存24小时和48小时，随后评估其活力、倍增时间、CD44和CD90抗原的表达、克隆/分化潜能以及核型。储存条件对eAd-MSCs活力有显著影响，而倍增时间没有显著改变。研究结果表明：（1）4°C储存优于室温储存，因为这会产生更多形态正常的活细胞；（2）细胞应在24小时内使用。
