Jarry J, Schadendorf D, Greenwood C, Spatz A, van Kempen L C
Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, Canada.
Department of Dermatology, University Hospital Essen, Germany.
Mol Oncol. 2014 Jun;8(4):819-29. doi: 10.1016/j.molonc.2014.02.009. Epub 2014 Mar 6.
MicroRNAs (miRNAs) in circulation have received an increasing amount of interest as potential minimal invasive diagnostic tools in oncology. Several diagnostic, prognostic and predictive signatures have been proposed for a variety of cancers at different stages of disease, but these have not been subjected to a critical review regarding their validity: reproducible identification in comparable studies and/or with different platforms of miRNA detection. In this review, we will critically address the results of circulating miRNA research in oncology that have been published between January 2008 and June 2013 (5.5 years), and discuss pre-analytical challenges, technological pitfalls and limitations that may contribute to the non-reproducibility of circulating miRNA research.
循环中的微小RNA(miRNA)作为肿瘤学中潜在的微创诊断工具,已越来越受到关注。针对不同疾病阶段的多种癌症,已经提出了几种诊断、预后和预测标志物,但这些标志物的有效性尚未经过严格审查:即在可比研究中和/或使用不同的miRNA检测平台进行可重复鉴定。在本综述中,我们将严格审视2008年1月至2013年6月(5.5年)间发表的肿瘤学中循环miRNA研究结果,并讨论可能导致循环miRNA研究不可重复性的分析前挑战、技术缺陷和局限性。