García-Rodríguez S, Arias-Santiago S, Orgaz-Molina J, Magro-Checa C, Valenzuela I, Navarro P, Naranjo-Sintes R, Sancho J, Zubiaur M
Departamento de Biología Celular e Inmunología, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN, Consejo Superior de Investigaciones Científicas (CSIC), Parque Tecnológico Ciencias de la Salud, Armilla, Granada, España.
Departamento de Dermatología, Hospital Universitario San Cecilio (HCUSC), Granada, España; Departamento de Dermatología, Hospital Virgen de las Nieves, Granada, España.
Actas Dermosifiliogr. 2014 Jun;105(5):497-503. doi: 10.1016/j.ad.2013.11.010. Epub 2014 Mar 20.
Circulating microRNAs (miRNA) are involved in the posttranscriptional regulation of genes associated with lipid metabolism (miRNA-33) and vascular function and angiogenesis (miRNA-126). The objective of this exploratory study was to measure plasma levels of miRNA-33 and miRNA-126 in patients with plaque psoriasis and evaluate their association with clinical parameters.
We studied 11 patients with plaque psoriasis. The median Psoriasis Area Severity Index (PASI) was 13 (interquartile range [IQR], 9-14) and body surface area involvement was 12 (IQR, 11-15). Eleven healthy controls matched for age and sex were also included. We analyzed cardiovascular risk factors and subclinical carotid atheromatosis. Plasma miRNAs were evaluated using quantitative real-time polymerase chain reaction.
Carotid intima-media thickness was greater in patients (0.57mm; IQR, 0.54-0.61; n=11) than in controls (0.50mm; IQR, 0.48-0.54; data available for 9 controls) (P=.0055, Mann-Whitney). Expression of miRNA-33 in patients (5.34; IQR, 3.12-7.96; n=11) was significantly higher than in controls (2.33; IQR, 1.71-2.84; only detected in 7 of 11 controls) (P=.0049, Wilcoxon signed rank). No differences in miRNA-126 levels were observed between patients and controls. In patients (n=11), we observed a positive correlation between miRNA-33 and insulin levels (r=0.7289, P=.0109) and a negative correlation between miRNA-126 and carotid intima-media thickness (r=-0.6181, P=.0426).
In psoriasis patients plasma levels of lipid and glucose metabolism-related miRNA-33 are increased and correlated with insulin. The study of circulating miRNA-33 in psoriasis may provide new insights about the associated systemic inflammatory abnormalities.
循环微RNA（miRNA）参与脂质代谢相关基因（miRNA - 33）以及血管功能和血管生成相关基因（miRNA - 126）的转录后调控。本探索性研究的目的是测定斑块状银屑病患者血浆中miRNA - 33和miRNA - 126的水平，并评估它们与临床参数的相关性。
我们研究了11例斑块状银屑病患者。银屑病面积和严重程度指数（PASI）中位数为13（四分位间距[IQR]，9 - 14），体表面积受累情况为12（IQR，11 - 15）。还纳入了11名年龄和性别匹配的健康对照者。我们分析了心血管危险因素和亚临床颈动脉粥样硬化。使用定量实时聚合酶链反应评估血浆miRNA。
患者的颈动脉内膜中层厚度（0.57mm；IQR，0.54 - 0.61；n = 11）大于对照组（0.50mm；IQR，0.48 - 0.54；9名对照者的数据可用）（P = 0.0055，曼 - 惠特尼检验）。患者中miRNA - 33的表达（5.34；IQR，3.12 - 7.96；n = 11）显著高于对照组（2.33；IQR，1.71 - 2.84；仅在11名对照者中的7名中检测到）（P = 0.0049，威尔科克森符号秩检验）。患者与对照组之间未观察到miRNA - 126水平的差异。在患者（n = 11）中，我们观察到miRNA - 33与胰岛素水平呈正相关（r = 0.7289，P = 0.0109），miRNA - 126与颈动脉内膜中层厚度呈负相关（r = -0.6181，P = 0.0426）。
在银屑病患者中，与脂质和葡萄糖代谢相关的miRNA - 33血浆水平升高且与胰岛素相关。对银屑病患者循环miRNA - 33的研究可能为相关的全身炎症异常提供新的见解。
