Department of Anatomy and Embryology, Faculty of Medicine, Beni Suef University, Salah Salem Street, Beni Suef 62511, Egypt.
Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Acta Histochem. 2014 Jun;116(5):844-54. doi: 10.1016/j.acthis.2014.02.002. Epub 2014 Mar 20.
Medicinal plants attract growing interest in the therapeutic management of Diabetes mellitus. Moringa oleifera is a remarkably nutritious vegetable with several antioxidant properties. The present study assessed the possible antioxidant and antidiabetic effects of an aqueous extract of M. oleifera leaves in treating streptozotocin-induced diabetic albino rats. The antidiabetic effects of aqueous extract of M. oleifera leaves were assessed histomorphometrically, ultrastructurally and biochemically. Fasting plasma glucose (FPG) was monitored and morphometric measurements of β-cells of islets of Langerhans (modified Gomori's stain) and collagen fibers (Mallory's trichrome stain) were performed. The antioxidant effects of M. oleifera leaves were determined by measuring the reduced glutathione and lipid peroxidation product, malondialdehyde, in pancreatic tissue. M. oleifera treatment significantly ameliorated the altered FPG (from 380% to 145%), reduced glutathione (from 22% to 73%) and malondialdehyde (from 385% to 186%) compared to control levels. The histopathological damage of islet cells was also markedly reversed. Morphometrically, M. oleifera significantly increased the areas of positive purple modified Gomori stained β-cells (from 60% to 91%) and decreased the area percentage of collagen fibers (from 199% to 120%) compared to control values. Experimental findings clearly indicate the potential benefits of using the aqueous extract of M. oleifera leaves as a potent antidiabetic treatment.
药用植物在糖尿病的治疗管理中引起了越来越多的关注。辣木是一种营养丰富的蔬菜，具有多种抗氧化特性。本研究评估了辣木叶水提物在治疗链脲佐菌素诱导的糖尿病白化大鼠中的潜在抗氧化和抗糖尿病作用。通过组织形态计量学、超微结构和生物化学评估辣木叶水提物的抗糖尿病作用。监测空腹血糖（FPG），并对胰岛β细胞的形态计量学测量（改良的 Gomori 染色）和胶原纤维（Mallory 三色染色）进行测量。通过测量胰腺组织中的还原型谷胱甘肽和脂质过氧化产物丙二醛来确定辣木叶的抗氧化作用。与对照组相比，辣木叶治疗显著改善了异常的 FPG（从 380%降至 145%）、还原型谷胱甘肽（从 22%降至 73%）和丙二醛（从 385%降至 186%）。胰岛细胞的组织病理学损伤也明显逆转。形态计量学上，与对照组相比，辣木叶显著增加了阳性紫色改良 Gomori 染色β细胞的面积（从 60%增加到 91%），并减少了胶原纤维的面积百分比（从 199%减少到 120%）。实验结果清楚地表明，使用辣木叶水提物作为一种有效的抗糖尿病治疗具有潜在的益处。
