Gall D, Colling A, Marino O, Moreno E, Nielsen K, Perez B, Samartino L
Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P9.
Clin Diagn Lab Immunol. 1998 Sep;5(5):654-61. doi: 10.1128/CDLI.5.5.654-661.1998.
The results of a field trial conducted in Latin America with two indirect enzyme-linked immunosorbent assays (ELISAs) and two competitive ELISAs (CELISAs) for the detection of bovine antibody to Brucella abortus are reported. One of the CELISA formats performed most accurately. The percentage of positive reactions in the CELISA relative to the selected positive rose bengal agglutination test (RBT) and complement fixation test (CFT) results was 97.47%, the percentage of negatives relative to the selected negative RBT and CFT results for unexposed cattle was 98.32%, and the percentage of negatives in cattle vaccinated with B. abortus 19 was 96.51%. The same assay format under Canadian conditions had an actual sensitivity of 100%, a specificity of 99.90% in nonvaccinates, and a specificity of 97.7% in a strain 19-vaccinated population. Overall, the CELISA performed as expected and the results were not dissimilar from the results obtained in the Canadian study. This provided further evidence that this CELISA can in many instances differentiate infected cattle from those that are vaccinated or infected with a cross-reacting organism while still giving very few false-positive or false-negative results.
报告了在拉丁美洲进行的一项田间试验结果，该试验采用两种间接酶联免疫吸附测定法（ELISA）和两种竞争ELISA法（CELISA）检测牛抗布鲁氏菌流产株抗体。其中一种CELISA形式表现最为准确。相对于选定的阳性玫瑰红平板凝集试验（RBT）和补体结合试验（CFT）结果，CELISA中的阳性反应百分比为97.47%；相对于选定的未接触牛的阴性RBT和CFT结果，阴性百分比为98.32%；在接种布鲁氏菌流产株19的牛中，阴性百分比为96.51%。在加拿大条件下，相同的检测形式实际灵敏度为100%，在未接种疫苗的牛中特异性为99.90%，在接种菌株19的牛群中特异性为97.7%。总体而言，CELISA表现符合预期，结果与加拿大研究所得结果并无不同。这进一步证明，这种CELISA在许多情况下能够区分感染牛与接种疫苗或感染交叉反应性生物体的牛，同时仍能产生极少的假阳性或假阴性结果。
