阿根廷猪布鲁氏菌病初步血清学诊断的主要结合试验评估
Evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in Argentina.
作者信息
Paulo P S, Vigliocco A M, Ramondino R F, Marticorena D, Bissi E, Briones G, Gorchs C, Gall D, Nielsen K
机构信息
Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina.
出版信息
Clin Diagn Lab Immunol. 2000 Sep;7(5):828-31. doi: 10.1128/CDLI.7.5.828-831.2000.
An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98. 3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.
使用两类猪血清对用于猪布鲁氏菌病血清学诊断的间接酶联免疫吸附测定（IELISA）、竞争酶联免疫吸附测定（CELISA）和荧光偏振测定（FPA）进行了评估：来自加拿大无布鲁氏菌病猪群的血清，以及根据缓冲抗原平板凝集试验（BPAT）和2-巯基乙醇（2-ME）试验中的阳性反应从阿根廷挑选的血清。此外，还对来自每个原产猪场至少分离出一次猪布鲁氏菌的成年猪血清进行了评估。IELISA、CELISA和FPA的特异性值分别为99.9%、99.5%和98.3%，相对于BPAT和2-ME试验，IELISA、CELISA和FPA的灵敏度值分别为98.9%、96.6%和93.8%。通过使用从培养出猪布鲁氏菌的个体猪采集的37份血清评估实际灵敏度，得到的值如下：BPAT为86.5%；2-ME试验为81.1%；IELISA为86.5%；CELISA为78.5%；FPA为80.0%。
Zotero 插件