Garcia Valdir Gouveia, Sahyon Angelita Strazzi, Longo Mariéllen, Fernandes Leandro Araújo, Gualberto Junior Erivan Clementino, Novaes Vivian Cristina Noronha, Ervolino Edilson, de Almeida Juliano Milanezi, Theodoro Letícia Helena
Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil; Department of Periodontics, University Center of the Educational Foundation of Barretos (UNIFEB), Barretos, SP, Brazil.
Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
J Craniomaxillofac Surg. 2014 Oct;42(7):1196-202. doi: 10.1016/j.jcms.2014.02.008. Epub 2014 Feb 25.
The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) on the bone repair of critical size defects (CSDs) filled with autogenous bone in the calvaria of immunosuppressed rats. A 5 mm-diameter CSD was created in the calvaria of 30 rats. The animals were divided into 5 groups (n = 6): Control (C)--the defect was filled with a blood clot; Dexamethasone (D)--dexamethasone treatment, and the defect was filled with a blood clot; Autogenous bone (AB)--dexamethasone treatment, and the defect was filled with autogenous bone; LLLT--dexamethasone treatment, and the defect received LLLT (660 nm; 35 mW; 24.7 J/cm(2)); and AB + LLLT--dexamethasone treatment, and the defect was filled with autogenous bone and received LLLT. All animals were euthanized at 30 postoperative days. Histometric and histological analyses were performed. The new bone area (NBA) was calculated as the percentage of the total area of the original defect. Data were analysed statistically (an analysis of variance and Tukey's test; P < 0.05). The AB + LLLT group showed the largest NBA of all groups (P < 0.05). The use of LLLT with AB effectively stimulated bone formation in CSDs in the calvaria of immunosuppressed rats.
本研究旨在评估低强度激光疗法（LLLT）对免疫抑制大鼠颅骨中填充自体骨的临界尺寸骨缺损（CSD）骨修复的影响。在30只大鼠的颅骨上制作直径为5毫米的CSD。将动物分为5组（n = 6）：对照组（C）——缺损处填充血凝块；地塞米松组（D）——地塞米松治疗，缺损处填充血凝块；自体骨组（AB）——地塞米松治疗，缺损处填充自体骨；LLLT组——地塞米松治疗，缺损处接受LLLT（660纳米；35毫瓦；24.7焦/平方厘米）；AB + LLLT组——地塞米松治疗，缺损处填充自体骨并接受LLLT。所有动物在术后30天安乐死。进行了组织计量学和组织学分析。新骨面积（NBA）计算为原始缺损总面积的百分比。对数据进行统计学分析（方差分析和Tukey检验；P < 0.05）。AB + LLLT组在所有组中显示出最大的NBA（P < 0.05）。LLLT与AB联合使用有效地刺激了免疫抑制大鼠颅骨CSD中的骨形成。
