碱性成纤维细胞生长因子的密度梯度引导血管平滑肌细胞的定向迁移。
A density gradient of basic fibroblast growth factor guides directional migration of vascular smooth muscle cells.
作者信息
Wu Jindan, Mao Zhengwei, Han Lulu, Zhao Yizhi, Xi Jiabin, Gao Changyou
机构信息
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China; MOE Key Laboratory of Advanced Textile Materials & Manufacturing Technology, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018, China.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
出版信息
Colloids Surf B Biointerfaces. 2014 May 1;117:290-5. doi: 10.1016/j.colsurfb.2014.02.043. Epub 2014 Mar 2.
The migration of vascular smooth muscle cells (VSMCs) is an important process in many physiological events. It is of paramount importance to control the migration rate and direction of VSMCs by biomaterials. In this paper, a density gradient of basic fibroblast growth factor (bFGF) was fabricated using an injection method and the bio-conjugation between heparin and bFGF. The density of bFGF gradually increased with a slope of 17 ng/cm(2)/mm. Adhesion and migration of VSMCs were studied on the bFGF gradient. The VSMCs exhibited preferential orientation and an enhanced directional migration behavior on the gradient surface. Up to 70% cells migrated towards the region with a higher density of bFGF on the gradient. However, the bFGF gradient had no effect on the cell migration rate.
血管平滑肌细胞（VSMCs）的迁移是许多生理过程中的一个重要过程。通过生物材料控制VSMCs的迁移速率和方向至关重要。在本文中，利用注射法以及肝素与碱性成纤维细胞生长因子（bFGF）之间的生物共轭作用构建了bFGF的密度梯度。bFGF的密度以17 ng/cm(2)/mm的斜率逐渐增加。研究了VSMCs在bFGF梯度上的黏附与迁移情况。VSMCs在梯度表面呈现出优先取向和增强的定向迁移行为。在梯度上，高达70%的细胞向bFGF密度较高的区域迁移。然而，bFGF梯度对细胞迁移速率没有影响。
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