Grewal Dilraj S, Chou Jonathan, Rollins Stuart D, Fawzi Amani A
Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.
PLoS One. 2014 Mar 21;9(3):e92841. doi: 10.1371/journal.pone.0092841. eCollection 2014.
To analyze the topographic correlation between reticular pseudodrusen (RPD) visualized on infrared reflectance (IR) and choroidal vasculature using en-face volumetric spectral-domain optical coherence tomography (SD-OCT).
A masked observer marked individual RPD on IR images using ImageJ (NIH, Bethesda, MD). Using the macular volume scan (Cirrus, Carl Zeiss Meditec Inc, Dublin, CA), the RPE slab function was used to generate a C-scan of the most superficial choroidal vasculature. An independent masked grader created a topographic binary map of the choroidal vasculature by thresholding the en-face image, which was overlaid onto the IR map of RPD. For each IR image, ImageJ was used to generate a random set of dots as "control lesions".
17 eyes of 11 patients (78±13.7 years) with RPD were analyzed. The average number of RPD lesions identified on IR images was 414±71.5, of which 49.6±4.3% were located overlying the choroidal vasculature, compared to 45.4±4.0% in controls (p = 0.014). 50.4±4.3% of lesions overlay the choroidal stroma, of which 76.5±3.1% were ≤3 pixels from the choroidal vessels. The percentage of RPD lesions located within ≤3 pixels from the choroidal vasculature was significantly greater than the percentage located ≥7 pixels away. (p<0.0001). Compared to controls (71.6±3.8%), RPD were more likely to be located ≤3 pixels away from choroidal vessels (p = 0.014). In contrast, control lesions were more likely to be ≥7 pixels away from choroidal vessels than RPD (9.1±1.9% vs. 4.8±1.2%, respectively, p = 0.002).
Our analysis shows that RPD lesions follow the underlying choroidal vasculature. Approximately half the RPD directly overlay the choroidal vessels and the majority of the remaining lesions were ≤3 pixels (≤30 microns) from the vessel edge, supporting the hypothesis that RPD maybe related to pathologic changes at the choroidal level.
使用正面容积光谱域光学相干断层扫描（SD-OCT）分析红外反射（IR）下可视化的网状假性玻璃膜疣（RPD）与脉络膜血管系统之间的地形相关性。
一名蒙面观察者使用ImageJ（美国国立卫生研究院，马里兰州贝塞斯达）在IR图像上标记单个RPD。使用黄斑容积扫描（Cirrus，卡尔蔡司医疗技术公司，加利福尼亚州都柏林），RPE平板功能用于生成最浅表脉络膜血管系统的C扫描。一名独立的蒙面分级员通过对正面图像进行阈值处理创建脉络膜血管系统的地形二元图，该图叠加在RPD的IR图上。对于每张IR图像，使用ImageJ生成一组随机点作为“对照病变”。
分析了11例（78±13.7岁）患有RPD的患者的17只眼睛。在IR图像上识别出的RPD病变的平均数量为414±71.5个，其中49.6±4.3%位于脉络膜血管系统上方，而对照组为45.4±4.0%（p = 0.014）。50.4±4.3%的病变覆盖脉络膜基质，其中76.5±3.1%距离脉络膜血管≤3个像素。位于距脉络膜血管系统≤3个像素内的RPD病变百分比显著大于位于≥7个像素外的百分比（p<0.0001）。与对照组（71.6±3.8%）相比，RPD更有可能位于距脉络膜血管≤3个像素处（p = 0.014）。相比之下，对照病变比RPD更有可能距离脉络膜血管≥7个像素（分别为9.1±1.9%和4.8±1.2%，p = 0.002）。
我们的分析表明，RPD病变遵循潜在的脉络膜血管系统。大约一半的RPD直接覆盖脉络膜血管，其余大多数病变距离血管边缘≤3个像素（≤30微米），支持RPD可能与脉络膜水平的病理变化有关的假设。
