Neumann H A, Herrmann D B, Fiebig H H, Engelhardt R
Medizinische Klinik der Ruhr Universität, St Josef Hospital, Bochum, F.R.G.
Eur J Cancer Clin Oncol. 1989 Jan;25(1):99-104. doi: 10.1016/0277-5379(89)90056-4.
Tumor cells derived from 13 different individual human tumors were plated in a colony forming monolayer assay. The effect of bleomycin and peplomycin on colony formation was assessed in normothermic conditions and after a hyperthermic treatment at 40.5 degrees C for 2 h at the beginning of the culture. In three out of the 13 tumor samples (two colon carcinomas, one malignant melanoma), hyperthermic incubation resulted in a thermal enhancement of the effects of bleomycin and peplomycin. In addition, human bone marrow progenitor cells (CFU-C) were subjected to the same procedure. Peplomycin proved to be less toxic to CFU-C than bleomycin. In samples from eight different donors, homogeneous dose-response curves were observed. There was no difference between normo- and hyperthermic incubation.
将源自13种不同个体人类肿瘤的肿瘤细胞接种于集落形成单层试验中。在常温条件下以及在培养开始时于40.5摄氏度进行2小时热疗后，评估博来霉素和培洛霉素对集落形成的影响。在13个肿瘤样本中的3个（2个结肠癌、1个恶性黑色素瘤）中，热孵育导致博来霉素和培洛霉素的作用出现热增强。此外，对人骨髓祖细胞（CFU-C）进行相同程序处理。结果表明，培洛霉素对CFU-C的毒性低于博来霉素。在来自8个不同供体的样本中，观察到了均匀的剂量反应曲线。常温孵育和热孵育之间没有差异。
