Neumann H A, Fiebig H H, Löhr G W, Engelhardt R
Eur J Cancer Clin Oncol. 1985 Apr;21(4):515-23. doi: 10.1016/0277-5379(85)90046-x.
A tumor colony-forming assay was used to investigate the effect of 40.5 degrees C hyperthermia and drugs on the colony-forming ability of human clonogenic tumor cells. In order to be able to perform repeated incubations with identical tumor material, specimens were used that were augmented in the nude mouse system. Five tumor samples (two malignant melanomas, two squamous cell carcinomas of the lung and one small cell carcinoma of the lung) were incubated with seven drugs: doxorubicin, actinomycin-D, bleomycin, melphalan, vincristine, vinblastin and cisplatinum. One additional tumor specimen (chondrosarcoma) was incubated with doxorubicin immediately after resection. The incubation with drugs at 37 degrees C revealed dose response curves typical for each tumor. Incubation at 40.5 degrees C for 2 hr showed enhanced drug effects in five out of the six tumors tested. The drugs, with an enhanced effect and the pattern of enhancement were different in each individual tumor.
采用肿瘤集落形成试验来研究40.5摄氏度热疗及药物对人克隆源性肿瘤细胞集落形成能力的影响。为了能够对相同的肿瘤材料进行重复培养，使用了在裸鼠系统中增殖的标本。五个肿瘤样本（两个恶性黑色素瘤、两个肺鳞状细胞癌和一个肺小细胞癌）与七种药物一起培养：阿霉素、放线菌素-D、博来霉素、美法仑、长春新碱、长春花碱和顺铂。另外一个肿瘤标本（软骨肉瘤）在切除后立即与阿霉素一起培养。在37摄氏度下与药物一起培养显示出每种肿瘤典型的剂量反应曲线。在40.5摄氏度下培养2小时，在所测试的六个肿瘤中有五个显示出增强的药物效果。每种肿瘤中效果增强的药物及其增强模式各不相同。
