Sobczyńska-Rak Aleksandra, Polkowska Izabela, Silmanowicz Piotr
University of Life Sciences in Lublin Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine Lublin Poland.
Acta Vet Hung. 2014 Sep;62(3):362-71. doi: 10.1556/AVet.2014.009.
Angiogenesis plays an essential role in the development of a neoplastic tumour by conditioning both its growth and the formation of metastases. The induction of blood vessel growth occurs under the influence of proangiogenic factors, among which Vascular Endothelial Growth Factor (VEGF) seems to be the most important. The aim of this research was to study the level of VEGF measured by ELISA in the serum of dogs with neoplasms of the oral cavity. The study material comprised samples of neoplastic tissue from 17 operated dogs and the serum of the examined animals as well as of dogs from the control group. The tissue samples were taken from dogs of different breeds, aged 6-14 years. The tumour type was determined in accordance with the applicable WHO classification. Blood samples taken from sick dogs and from animals of the control group were centrifuged, and immunoenzymatic labelling of VEGF was performed in the obtained serum using ELISA and R&D system reagents (Quantikine Canine VEGF). All stages of VEGF labelling were performed according to the recommendation of the test manufacturer. The median of VEGF in the serum of the dogs with neoplasms of the oral cavity was 40.64 pg/mL. The lowest value of 14.26 pg/mL was observed in the case of fibrosarcoma, and the highest value of 99.19 pg/mL in the case of squamous cell carcinoma. The VEGF median in the control group amounted to 11.14 pg/mL whereas the VEGF value in the groups of animals diagnosed with benign tumours ranged between 2.30 and 19.74 pg/mL. Elevated VEGF in the blood serum, in comparison with the benign tumour group and the control group, was observed in all examined neoplasms of the oral cavity. It was suggested that overexpression of VEGF can have a prognostic value and is useful in the early detection of neoplasms.
血管生成在肿瘤的发展过程中起着至关重要的作用，它既影响肿瘤的生长，也影响转移灶的形成。血管生长的诱导发生在促血管生成因子的影响下，其中血管内皮生长因子（VEGF）似乎是最重要的。本研究的目的是通过酶联免疫吸附测定法（ELISA）研究患有口腔肿瘤的犬血清中VEGF的水平。研究材料包括17只接受手术的犬的肿瘤组织样本、被检查动物以及对照组犬的血清。组织样本取自6至14岁不同品种的犬。肿瘤类型根据适用的世界卫生组织分类确定。对患病犬和对照组动物采集的血样进行离心，使用ELISA和R&D系统试剂（Quantikine Canine VEGF）在获得的血清中进行VEGF的免疫酶标记。VEGF标记的所有阶段均按照测试制造商的建议进行。患有口腔肿瘤的犬血清中VEGF的中位数为40.64 pg/mL。在纤维肉瘤病例中观察到最低值为14.26 pg/mL，在鳞状细胞癌病例中观察到最高值为99.19 pg/mL。对照组中VEGF的中位数为11.14 pg/mL，而被诊断为良性肿瘤的动物组中VEGF值在2.30至19.74 pg/mL之间。与良性肿瘤组和对照组相比，在所有检查的口腔肿瘤中均观察到血清中VEGF升高。有人认为VEGF的过表达可能具有预后价值，并且有助于肿瘤的早期检测。
