Nasry Walaa Hamed Shaker, Rodriguez-Lecompte Juan Carlos, Martin Chelsea K
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada.
J Vet Diagn Invest. 2025 Mar;37(2):223-233. doi: 10.1177/10406387251315677. Epub 2025 Feb 10.
Feline oral squamous cell carcinoma (FOSCC) is an aggressive tumor with poor outcomes. Mechanisms of prostaglandin E2 (PGE2)-related inflammation and angiogenesis interact in human OSCC; however, this relationship has not been reported in FOSCC, to our knowledge. We aimed to characterize expression of genes encoding PGE2 synthases (), PGE2 receptors (), hypoxia inducible factor 1α (), and vascular and endothelial growth factor A () in FOSCC cell lines (SCCF1-3) in vitro using reverse-transcription quantitative real-time PCR (RT-qPCR). Expression of , , , and were serum-inducible in SCCF2 cells; was also inducible in SCCF1 cells ( ≤ 0.05). Compared to other serum-treated cells, SCCF3 cells had the lowest expression despite the highest ( ≤ 0.05) expression. PGE2 (5 µg/mL and 35 µg/mL) was added to SCCF2 cells for 4 different times (30, 60, 120, 240 min). Both doses of PGE2 stimulated expression of and at 240 min ( ≤ 0.05). PGE2 treatment stimulated cyclooxygenase 2 () expression at 30 min, followed by suppression at 60 and 120 min and a sharp reduction in expression at 60 min ( ≤ 0.05). Treatment of SCCF2 with PGE2 and EP4 antagonist L-161,982 increased expression, and L-161,982 (alone and in combination with PGE2) stimulated expression ( ≤ 0.05). Genes for PGE2 synthase enzymes, PGE2 receptors, HIF1α and VEGFA were expressed in FOSCC cells in vitro. SCCF2 cells responded to exogenous PGE2 and EP4 antagonism, suggesting that EP4 activity in FOSCC deserves more study.
猫口腔鳞状细胞癌（FOSCC）是一种侵袭性肿瘤，预后较差。前列腺素E2（PGE2）相关炎症和血管生成的机制在人类口腔鳞状细胞癌（OSCC）中相互作用；然而，据我们所知，这种关系在FOSCC中尚未见报道。我们旨在使用逆转录定量实时PCR（RT-qPCR）在体外对FOSCC细胞系（SCCF1-3）中编码PGE2合成酶（）、PGE2受体（）、缺氧诱导因子1α（）以及血管内皮生长因子A（）的基因表达进行表征。在SCCF2细胞中，、、和的表达受血清诱导；在SCCF1细胞中也可诱导表达（≤0.05）。与其他血清处理的细胞相比，尽管SCCF3细胞的表达最高（≤0.05），但其表达最低。将PGE2（5μg/mL和35μg/mL）添加到SCCF2细胞中，处理4个不同时间（30、60、120、240分钟）。两种剂量的PGE2在240分钟时均刺激了和的表达（≤0.05）。PGE2处理在30分钟时刺激了环氧合酶2（）的表达，随后在60和120分钟时受到抑制，在60分钟时表达急剧下降（≤0.05）。用PGE2和EP4拮抗剂L-161,982处理SCCF2可增加表达，L-161,982（单独以及与PGE2联合使用）刺激了表达（≤0.05）。体外FOSCC细胞中表达了PGE2合成酶、PGE2受体、HIF1α和VEGFA的基因。SCCF2细胞对外源性PGE2和EP4拮抗有反应，这表明FOSCC中的EP4活性值得进一步研究。
