Chen W, Gendrault J L, Steffan A M, Jeandidier E, Kirn A
Laboratoire de Virologie, Faculté de Médecine de Strasbourg, France.
Hepatology. 1989 Mar;9(3):352-62. doi: 10.1002/hep.1840090303.
Fat-storing cells were isolated from 15-day-old mouse sinusoidal cell cultures (Kupffer or endothelial cells), where they had multiplied abundantly; they were then purified by a negative selection method based on the fact that they do not possess Fc receptors, as do both other types of cells. The fat-storing cells, which could be subcultured for at least 10 passages, have the main morphological characteristics already described in vivo, in particular, the rough endoplasmic reticulum and the lipid droplets, which become less and less apparent as the number of passages increases. Subcultured fat-storing cells, almost devoid of lipid droplets and vitamin A, were able to take up retinol, as the appearance of a typical autofluorescence indicated; the number of lipid droplets increased concomitantly. Furthermore, the cultured fat-storing cells were able to internalize one-micron-sized latex beads by phagocytosis. Infection of fat-storing cells with mouse hepatitis virus 3, ectromelia or Sindbis virus led to multiplication of the virus particles. There was a direct relation between the multiplication of mouse hepatitis virus 3 in cultured fat-storing cells and the susceptibility of the animals to the virus. In the case of Sindbis virus, interferon is produced, its production being independent of the presence of vitamin A.
储脂细胞是从15日龄小鼠的肝血窦细胞培养物（库普弗细胞或内皮细胞）中分离出来的，在这些培养物中它们大量增殖；然后基于它们不像其他两种细胞那样具有Fc受体这一事实，通过负选法对其进行纯化。能够传代培养至少10代的储脂细胞具有体内已描述的主要形态特征，特别是粗面内质网和脂滴，随着传代次数增加，脂滴变得越来越不明显。传代培养的储脂细胞几乎没有脂滴和维生素A，但能够摄取视黄醇，这通过典型自发荧光的出现得以表明；脂滴数量随之增加。此外，培养的储脂细胞能够通过吞噬作用内化1微米大小的乳胶珠。用小鼠肝炎病毒3、痘苗病毒或辛德毕斯病毒感染储脂细胞会导致病毒颗粒增殖。小鼠肝炎病毒3在培养的储脂细胞中的增殖与动物对该病毒的易感性之间存在直接关系。就辛德毕斯病毒而言，会产生干扰素，其产生与维生素A的存在无关。
