小鼠肝炎病毒3的毒力，由培养的肝细胞对逃逸突变体的易感性所证明。
The virulence of mouse hepatitis virus 3, as evidenced by permissivity of cultured hepatic cells toward escape mutants.
作者信息
Martin J P, Chen W, Koehren F, Pereira C A
机构信息
Unité INSERM U74, Université Louis Pasteur, Strasbourg, France.
出版信息
Res Virol. 1994 Sep-Oct;145(5):297-302. doi: 10.1016/s0923-2516(07)80034-3.
Abstract
Deux mutants d'échappement à la neutralisation du MHV3 (MHV 51.6 et MHV Cl12) montrent, en comparaison au MHV3 sauvage (wt), un ralentissement de leur multiplication chez des souris sensibles BALB/c et une atténuation du pouvoir pathogène provoquant un retard considérable de la mortalité. Le MHV3 wt se réplique à des titres élevés dans toutes les cellules hépatiques en culture. Le MHV3 51.6, de virulence intermédiaire, se multiplie dans toutes les cellules hépatiques excepté dans les cellules endothéliales. Le mutant qui tue le plus tardivement les souris (MHV Cl12) se multiplie uniquement dans les cellules de Ito, les autres étant non permissives. Ces résultats montrent une corrélation directe entre la virulence du MHV3 et son aptitude à se répliquer de manière efficace dans les cellules hépatiques .
摘要
两种MHV3中和逃逸突变体（MHV 51.6和MHV Cl12）与野生型MHV3（wt）相比，在敏感的BALB/c小鼠中增殖速度减慢，致病力减弱，导致死亡率显著延迟。野生型MHV3在所有培养的肝细胞中都能以高滴度复制。中等毒力的MHV3 51.6在除内皮细胞外的所有肝细胞中增殖。致死小鼠最晚的突变体（MHV Cl12）仅在伊托细胞中增殖，其他细胞不支持其增殖。这些结果表明MHV3的毒力与其在肝细胞中有效复制的能力之间存在直接相关性。
相似文献
1
The virulence of mouse hepatitis virus 3, as evidenced by permissivity of cultured hepatic cells toward escape mutants.小鼠肝炎病毒3的毒力，由培养的肝细胞对逃逸突变体的易感性所证明。
Res Virol. 1994 Sep-Oct;145(5):297-302. doi: 10.1016/s0923-2516(07)80034-3.
2
Pathogenicity of neutralization escape mutants of mouse hepatitis virus: correlation with T- and B-cell depletions.小鼠肝炎病毒中和逃逸突变体的致病性：与T细胞和B细胞耗竭的相关性。
Res Immunol. 1994 Sep;145(7):553-65. doi: 10.1016/s0923-2494(94)80073-1.
3
Selective Packaging in Murine Coronavirus Promotes Virulence by Limiting Type I Interferon Responses.选择性包装促进鼠冠状病毒毒力通过限制 I 型干扰素反应。
mBio. 2018 May 1;9(3):e00272-18. doi: 10.1128/mBio.00272-18.
4
The severity of hepatic lesion after intraperitoneal JHMV infection in IFN-gamma deficient mice is parallel to viral replication in hepatocytes in vitro.在γ干扰素缺陷小鼠中，腹腔内感染JHMV后肝脏病变的严重程度与体外肝细胞中的病毒复制情况平行。
Adv Exp Med Biol. 2001;494:95-9. doi: 10.1007/978-1-4615-1325-4_15.
5
Organ specific endothelial cell heterogeneity influences differential replication and cytopathogenicity of MHV-3 and MHV-4. Implications in viral tropism.器官特异性内皮细胞异质性影响MHV-3和MHV-4的差异复制及细胞致病性。对病毒嗜性的影响。
Adv Exp Med Biol. 1995;380:43-50. doi: 10.1007/978-1-4615-1899-0_6.
6
Characterization of attenuated mutants of MHV3: importance of the E2 protein in organ tropism and infection of isolated liver cells.MHV3减毒突变体的特性：E2蛋白在器官嗜性和分离肝细胞感染中的重要性
Adv Exp Med Biol. 1990;276:403-10. doi: 10.1007/978-1-4684-5823-7_55.
7
Localization of neurovirulence determinant for rats on the S1 subunit of murine coronavirus JHMV.鼠冠状病毒JHMV S1亚基上大鼠神经毒力决定因素的定位
Virology. 1995 Apr 1;208(1):67-74. doi: 10.1006/viro.1995.1130.
8
Neurovirulence for mice of soluble receptor-resistant mutants of murine coronavirus JHMV.鼠冠状病毒JHMV可溶性受体抗性突变体对小鼠的神经毒力
Adv Exp Med Biol. 2001;494:145-8. doi: 10.1007/978-1-4615-1325-4_23.
9
Role of the spike protein in murine coronavirus induced hepatitis: an in vivo study using targeted RNA recombination.刺突蛋白在鼠冠状病毒诱导的肝炎中的作用：一项使用靶向RNA重组的体内研究
Adv Exp Med Biol. 2001;494:139-44. doi: 10.1007/978-1-4615-1325-4_22.
10
MHV-A59 fusion mutants are attenuated and display altered hepatotropism.MHV - A59融合突变体减毒且显示出改变的嗜肝性。
Virology. 1994 Apr;200(1):1-10. doi: 10.1006/viro.1994.1156.
引用本文的文献
1
Preclinical models of acute liver failure: a comprehensive review.急性肝衰竭的临床前模型：全面综述
PeerJ. 2021 Dec 9;9:e12579. doi: 10.7717/peerj.12579. eCollection 2021.
2
Depletion of RIPK1 in hepatocytes exacerbates liver damage in fulminant viral hepatitis.肝细胞中 RIPK1 的耗竭会加剧暴发性病毒性肝炎的肝损伤。
Cell Death Dis. 2019 Jan 8;10(1):12. doi: 10.1038/s41419-018-1277-3.
3
Mouse Hepatitis Virus Infection Induces a Toll-Like Receptor 2-Dependent Activation of Inflammatory Functions in Liver Sinusoidal Endothelial Cells during Acute Hepatitis.小鼠肝炎病毒感染在急性肝炎期间诱导肝窦内皮细胞中炎症功能的Toll样受体2依赖性激活。
J Virol. 2016 Sep 29;90(20):9096-113. doi: 10.1128/JVI.01069-16. Print 2016 Oct 15.
4
Toll-like receptor-2 exacerbates murine acute viral hepatitis.Toll样受体2加剧小鼠急性病毒性肝炎。
Immunology. 2016 Oct;149(2):204-24. doi: 10.1111/imm.12627. Epub 2016 Aug 10.
5
Brain Invasion by Mouse Hepatitis Virus Depends on Impairment of Tight Junctions and Beta Interferon Production in Brain Microvascular Endothelial Cells.小鼠肝炎病毒入侵大脑取决于脑微血管内皮细胞紧密连接的损伤和β干扰素的产生。
J Virol. 2015 Oct;89(19):9896-908. doi: 10.1128/JVI.01501-15. Epub 2015 Jul 22.
6
A synergistic interferon-gamma production is induced by mouse hepatitis virus in interleukin-12 (IL-12)/IL-18-activated natural killer cells and modulated by carcinoembryonic antigen-related cell adhesion molecules (CEACAM) 1a receptor.鼠肝炎病毒在白细胞介素 12（IL-12）/白细胞介素 18 激活的自然杀伤细胞中诱导协同干扰素-γ产生，并受癌胚抗原相关细胞黏附分子（CEACAM）1a 受体调节。
Immunology. 2009 Sep;128(1 Suppl):e551-61. doi: 10.1111/j.1365-2567.2008.03030.x. Epub 2008 Dec 18.
7
Macrophage interleukin-6 and tumour necrosis factor-alpha are induced by coronavirus fixation to Toll-like receptor 2/heparan sulphate receptors but not carcinoembryonic cell adhesion antigen 1a.冠状病毒与 Toll 样受体 2/硫酸乙酰肝素受体结合可诱导巨噬细胞产生白细胞介素-6 和肿瘤坏死因子-α，但不能诱导癌胚细胞黏附抗原 1a。
Immunology. 2009 Sep;128(1 Suppl):e181-92. doi: 10.1111/j.1365-2567.2008.02946.x. Epub 2009 Aug 4.
8
Intrahepatic endothelial and Kupffer cells involved in immunosuppressive cytokines and natural killer (NK)/NK T cell disorders in viral acute hepatitis.肝内内皮细胞和库普弗细胞参与病毒性急性肝炎中免疫抑制细胞因子及自然杀伤（NK）/NK T细胞紊乱。
Clin Exp Immunol. 2008 May;152(2):298-310. doi: 10.1111/j.1365-2249.2008.03628.x. Epub 2008 Mar 10.
9
Recovery from mouse hepatitis virus infection depends on recruitment of CD8(+) cells rather than activation of intrahepatic CD4(+)alphabeta(-)TCR(inter) or NK-T cells.小鼠肝炎病毒感染的恢复取决于CD8(+)细胞的募集，而非肝内CD4(+)αβ(-)TCR(inter)或自然杀伤T细胞的激活。
Clin Immunol. 2001 Dec;101(3):345-56. doi: 10.1006/clim.2001.5131.
10
Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism.鼠冠状病毒A59型小鼠肝炎病毒刺突基因内的靶向重组：Q159是嗜肝性的一个决定因素。
J Virol. 1998 Dec;72(12):9628-36. doi: 10.1128/JVI.72.12.9628-9636.1998.
本文引用的文献
1
Production of monoclonal antibodies: strategy and tactics.单克隆抗体的制备：策略与战术
J Immunol Methods. 1980;35(1-2):1-21. doi: 10.1016/0022-1759(80)90146-5.
2
Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3.小鼠肝炎病毒A59：mRNA结构及与嗜肝性毒株MHV-3序列差异的基因定位
J Virol. 1981 Sep;39(3):823-34. doi: 10.1128/JVI.39.3.823-834.1981.
3
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.大鼠肝脏实质细胞分离的高效制备：生化与精细结构研究
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
4
Pathogenicity of antigenic variants of murine coronavirus JHM selected with monoclonal antibodies.用单克隆抗体筛选的鼠冠状病毒JHM抗原变异体的致病性
J Virol. 1986 Jun;58(3):869-75. doi: 10.1128/JVI.58.3.869-875.1986.
5
Site-specific alteration of murine hepatitis virus type 4 peplomer glycoprotein E2 results in reduced neurovirulence.4型鼠肝炎病毒纤突糖蛋白E2的位点特异性改变导致神经毒力降低。
J Virol. 1986 Aug;59(2):463-71. doi: 10.1128/JVI.59.2.463-471.1986.
6
Temperature-sensitive mutants of mouse hepatitis virus type 3 (MHV-3): isolation, biochemical and genetic characterization.3型小鼠肝炎病毒（MHV-3）的温度敏感突变体：分离、生化及遗传学特征分析
Arch Virol. 1988;100(3-4):147-60. doi: 10.1007/BF01487679.
7
Isolation, culture and main characteristics of mouse fat-storing cells: interaction with viruses.小鼠贮脂细胞的分离、培养及主要特性：与病毒的相互作用
Hepatology. 1989 Mar;9(3):352-62. doi: 10.1002/hep.1840090303.
8
The peplomer protein E2 of coronavirus JHM as a determinant of neurovirulence: definition of critical epitopes by variant analysis.冠状病毒JHM的包膜糖蛋白E2作为神经毒力的决定因素：通过变异分析确定关键表位
J Gen Virol. 1988 Jan;69 ( Pt 1):87-98. doi: 10.1099/0022-1317-69-1-87.
9
Characterization of attenuated mutants of MHV3: importance of the E2 protein in organ tropism and infection of isolated liver cells.MHV3减毒突变体的特性：E2蛋白在器官嗜性和分离肝细胞感染中的重要性
Adv Exp Med Biol. 1990;276:403-10. doi: 10.1007/978-1-4684-5823-7_55.
10
Increased susceptibility to mouse hepatitis virus type 3 (MHV3) infection induced by a hypercholesterolaemic diet with increased adsorption of MHV3 to primary hepatocyte cultures.高胆固醇饮食导致对3型小鼠肝炎病毒（MHV3）感染的易感性增加，并使MHV3对原代肝细胞培养物的吸附增加。
Res Virol. 1991 Jan-Feb;142(1):5-15. doi: 10.1016/0923-2516(91)90022-u.
Zotero 插件