Kawase T, Shiratori Y, Sugimoto T
Exp Cell Biol. 1986;54(4):183-92. doi: 10.1159/000163355.
Morphological changes, proliferation and collagen synthesis of fat-storing cells (FSC) in primary culture were examined. FSC, isolated from rats treated with vitamin A, showed numerous large lipid droplets in the cytoplasm and positive desmin staining. After 4-7 days culture, these cells were transformed into fibroblast-like cells with a gradual depletion of lipid droplets and with abundant well-developed rough endoplasmic reticulum. The proliferation analysis revealed that DNA synthesis preceded the increase of cell number. Enhancement of the collagen synthesis by FSC were associated with the morphological change of the cells. Quantitative analysis revealed that these cells produced mainly type I collagen (84%) and a small amount of type III collagen (16%).
研究了原代培养的贮脂细胞（FSC）的形态变化、增殖及胶原合成情况。从用维生素A处理的大鼠中分离出的FSC，其细胞质中有许多大脂滴，结蛋白染色呈阳性。培养4 - 7天后，这些细胞转变为成纤维细胞样细胞，脂滴逐渐减少，粗面内质网丰富且发达。增殖分析表明，DNA合成先于细胞数量增加。FSC胶原合成的增强与细胞的形态变化有关。定量分析显示，这些细胞主要产生I型胶原（84%）和少量III型胶原（16%）。
