Winzek C, Baumgärtel H
Institut für Physikalische und Theoretische Chemie, Freie Universität Berlin, Germany.
Histochemistry. 1988;90(1):73-7. doi: 10.1007/BF00495710.
The staining kinetics of single cells have been investigated using a perfusion cuvette in combination with a computer controlled microscope spectrometer. The physicochemical hydrodynamics of staining are characterized. Using a steady-state laminar flow parallel to the cell surface a hydrodynamic and a diffusional boundary layer are observed which are determined by the flow rate. The thickness of the diffusional boundary layer revealed by experimental data is in agreement with theoretically calculated values. At certain well-defined hydrodynamic conditions convective diffusion has no further effect on the staining rate.
使用灌注比色皿结合计算机控制的显微镜光谱仪对单细胞的染色动力学进行了研究。表征了染色的物理化学流体动力学。利用平行于细胞表面的稳态层流，观察到由流速决定的流体动力学边界层和扩散边界层。实验数据揭示的扩散边界层厚度与理论计算值一致。在某些明确的流体动力学条件下，对流扩散对染色速率没有进一步影响。
