Winzek C, Baumgärtel H
Institut für Physikalische und Theoretische Chemie, Freie Universität Berlin, Germany.
Histochemistry. 1988;90(1):79-83. doi: 10.1007/BF00495711.
Applying hydrodynamic conditions, which certify a negligible influence of convective diffusion, the time-dependent uptake of thionin in lymphocytes, monkey kidney cells, and their separated nuclei was measured spectroscopically. Using fixed cell material the dye transport inside the cell is not hindered due to plasma membrane and cytoplasm. The staining rate depends on the dye concentration, the pretreatment of the cell, and on the electrolyte concentration of the dye solution. The mechanism of dye migration inside the cell is in accordance with a porous matrix model. The diffusion process takes place inside the pores and channels filled with liquid and is modified by adsorption of dye molecules on the walls of the pores. A dynamic reversible equilibrium exists between migrating dye molecules and the binding sites on the pore walls described by the Freundlich adsorption isotherm. The proposed model explains the observed order of reaction of the staining kinetics.
在对流扩散影响可忽略不计的流体动力学条件下，用光谱法测量了硫堇在淋巴细胞、猴肾细胞及其分离细胞核中的时间依赖性摄取。使用固定的细胞材料时，由于质膜和细胞质的存在，细胞内的染料运输不受阻碍。染色速率取决于染料浓度、细胞的预处理以及染料溶液的电解质浓度。细胞内染料迁移的机制符合多孔基质模型。扩散过程发生在充满液体的孔和通道内，并因染料分子在孔壁上的吸附而改变。迁移的染料分子与孔壁上的结合位点之间存在动态可逆平衡，由弗罗因德利希吸附等温线描述。所提出的模型解释了染色动力学中观察到的反应顺序。
