He Yan, Ren Ling-Yan, Shan Ke-Ren, Zhang Ting, Wang Chan-Juan, Guan Zhi-Zhong
a Department of Molecular Biology and.
b Department of Pathology , Affiliated Hospital of Guiyang Medical University , Guiyang , P. R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):365-70. doi: 10.3109/19401736.2014.895990. Epub 2014 Mar 24.
To characterize the genetic profiles and relationships between ancient ethnic populations, we analyzed polymorphisms in mitochondrial DNA (mtDNA) isolated from the blood of 753 members of 12 ethnic groups (Buyi, Dong, Gelao, Hui, Man, Miao, Menggu, Mulao, Maonan, Qiang, She and Zhuang) living in the Guizhou Province of China. The 9-bp deletion of mtDNA was detected by the polymerase chain reaction (PCR) and PCR-PAGE, and 11 SNPs by restriction fragment length polymorphism and mini-sequencing. Thereafter, these genotyping results were verified by PCR-DNA sequencing. The mtDNA of these populations exhibited considerable diversity, both with respect to the haplogroups M and N, and subgroups thereof. The differences between the major ethnic groups reflected the maternal inheritance. These ethnic groups in Guizhou demonstrated a genetic profile that differed considerably from that of other Asian populations. Our findings indicate that the matrilineal genetic profiles of Guizhou groups are relatively complex and distinct, showing relationships that reflect national history and geography.
为了表征古代族群之间的基因特征和关系，我们分析了从居住在中国贵州省的12个民族（布依族、侗族、仡佬族、回族、满族、苗族、蒙古族、仫佬族、毛南族、羌族、畲族和壮族）的753名成员血液中分离出的线粒体DNA（mtDNA）的多态性。通过聚合酶链反应（PCR）和PCR-聚丙烯酰胺凝胶电泳（PAGE）检测mtDNA的9碱基缺失，并通过限制性片段长度多态性和微测序检测11个单核苷酸多态性（SNP）。此后，通过PCR-DNA测序验证这些基因分型结果。这些人群的mtDNA在单倍群M和N及其亚群方面都表现出相当大的多样性。主要民族之间的差异反映了母系遗传。贵州的这些民族表现出与其他亚洲人群有很大不同的基因特征。我们的研究结果表明，贵州各群体的母系基因特征相对复杂且独特，显示出反映民族历史和地理的关系。
