Liu Changlin, Li Yuan, Chen Siqing, Song Na, Gao Tianxiang
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Qingdao , P.R. China and.
Mitochondrial DNA. 2015 Aug;26(4):649-50. doi: 10.3109/19401736.2013.836518. Epub 2013 Oct 3.
The long PCR and primer walking methods are employed for determining the complete mitochondrial genome sequence of Pampus punctatissimus. The mitogenome is a circular molecule of 16,551 bp in length including the structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and two non-coding regions (L-strand replication origin and control region). Within the control region, we identify the termination-associated sequence domain (TAS), a central conserved domain (CSB-F, CSB-E and CSB-D), and three conserved sequence blocks (CSB-1, CSB-2 and CSB-3).
采用长PCR和引物步移法测定斑鰶的完整线粒体基因组序列。线粒体基因组是一个长度为16,551 bp的环状分子，包括13个蛋白质编码基因、两个核糖体RNA基因、22个转运RNA基因和两个非编码区（轻链复制起点和控制区）的结构。在控制区内，我们鉴定出终止相关序列结构域（TAS）、一个中央保守结构域（CSB-F、CSB-E和CSB-D）以及三个保守序列块（CSB-1、CSB-2和CSB-3）。
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