Li Yuan, Song Na, Gao Tianxiang
a Institute of Evolution and Marine Biodiversity, Ocean University of China , Qingdao , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):601-2. doi: 10.3109/19401736.2014.908365. Epub 2014 Apr 14.
Long PCR and primer walking methods are employed for determining the complete mitochondrial genome (mitogenome) sequence of Pampus chinensis. The mitogenome is a circular molecule of 16,534 bp in length including the structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions (L-strand replication origin and control region). Within the control region, we identify the termination-associated sequence domain (TAS), central conserved sequence block domains (CSB-F, CSB-E and CSB-D), and conserved sequence blocks (CSB-1, CSB-2 and CSB-3).
采用长PCR和引物步移法测定中华鲳线粒体基因组(mitogenome)序列。线粒体基因组是一个长度为16534 bp的环状分子,包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和2个非编码区(轻链复制起点和控制区)的结构。在控制区内,我们鉴定出终止相关序列结构域(TAS)、中央保守序列块结构域(CSB-F、CSB-E和CSB-D)以及保守序列块(CSB-1、CSB-2和CSB-3)。