健康儿童尿肾损伤生物标志物KIM-1和NGAL的参考区间
Reference intervals for urinary renal injury biomarkers KIM-1 and NGAL in healthy children.
作者信息
McWilliam Stephen J, Antoine Daniel J, Sabbisetti Venkata, Pearce Robin E, Jorgensen Andrea L, Lin Yvonne, Leeder J Steven, Bonventre Joseph V, Smyth Rosalind L, Pirmohamed Munir
机构信息
Department of Molecular & Clinical Pharmacology, University of Liverpool, Liverpool, Merseyside, UK.
出版信息
Biomark Med. 2014;8(10):1189-97. doi: 10.2217/bmm.14.36.
AIM
The aim of this study was to establish reference intervals in healthy children for two novel urinary biomarkers of acute kidney injury, kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL).
MATERIALS & METHODS: Urinary biomarkers were determined in samples from children in the UK (n = 120) and the USA (n = 171) using both Meso Scale Discovery (MSD) and Luminex-based analytical approaches.
RESULTS
95% reference intervals for each biomarker in each cohort are presented and stratified by sex or ethnicity where necessary, and age-related variability is explored using quantile regression. We identified consistently higher NGAL concentrations in females than males (p < 0.0001), and lower KIM-1 concentrations in African-Americans than Caucasians (p = 0.02). KIM-1 demonstrated diurnal variation, with higher concentrations in the morning (p < 0.001).
CONCLUSION
This is the first report of reference intervals for KIM-1 and NGAL using two analytical methods in a healthy pediatric population in both UK and US-based populations.
目的
本研究的目的是为急性肾损伤的两种新型尿液生物标志物——肾损伤分子-1（KIM-1）和中性粒细胞明胶酶相关脂质运载蛋白（NGAL），建立健康儿童的参考区间。
材料与方法
使用基于Meso Scale Discovery（MSD）和Luminex的分析方法，对来自英国（n = 120）和美国（n = 171）儿童的样本进行尿液生物标志物检测。
结果
给出了每个队列中每种生物标志物的95%参考区间，并在必要时按性别或种族进行分层，同时使用分位数回归探讨与年龄相关的变异性。我们发现女性的NGAL浓度始终高于男性（p < 0.0001），非裔美国人的KIM-1浓度低于白种人（p = 0.02）。KIM-1表现出昼夜变化，早晨浓度较高（p < 0.001）。
结论
这是在英国和美国健康儿科人群中使用两种分析方法得出的KIM-1和NGAL参考区间的首份报告。
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