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[The production of spread synaptonemal complexes of meiocytes from animals and plants by a drying method].

作者信息

Burd S B, Belousova G G, Klimenko V V

出版信息

Tsitol Genet. 1988 Nov-Dec;22(6):8-12.

PMID:2466355
Abstract

A spreading technique was used to prepare synaptonemal complexes from animals (Bombyx mori and Mus musculus spermatocytes) and plants (Zea mays microsporocytes). Suspension of hypotonically treated cells was spread over plastic-coated slides and air-dried. Fixed and phosphotungsten acid-stained spread preparations were studied by phase-contrast and electron microscopes. The spreading-through-drying method is more efficient and thereby more preferable as compared to the classical hypophase spreading technique.

摘要

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