Masuyama Hisashi, Hiramatsu Yuji
Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
PLoS One. 2014 Mar 24;9(3):e92805. doi: 10.1371/journal.pone.0092805. eCollection 2014.
Recent reports indicated that nutrition in early infancy might influence later child health outcomes such as obesity and metabolic syndrome. Therefore, we examined the effects of maternal high fat diet (HFD) during lactation on the onset of a metabolic syndrome in their offspring. All offspring were cross-fostered by dams on the same or opposite diet to yield 4 groups: offspring from HFD-fed dams suckled by HFD-fed dams (OHH) and by control diet (CD)-fed dams (OHC) and CD-fed dams suckled by HFD-fed dams (OCH) and by CD-fed dams (OCC) mice. We examined several metabolic syndrome-related factors including body weight, blood pressure, glucose tolerance and adipocytokines. Mean body weights of OHH and OCH mice were significantly higher than those of OHC and OCC mice, respectively, with elevated systolic blood pressure. Moreover, OHH and OCH mice revealed significantly worse glucose tolerance compared with the OHC and OCC mice, respectively. Triglyceride and leptin levels were significantly increased and adiponectin levels were significantly reduced by the maternal HFD during lactation, with similar changes in leptin and adiponectin mRNA expression but without histone modifications in adipose tissues. In addition, maternal obesity induced by HFD during lactation increased and prolonged the leptin surge in the offspring and the gender differences of leptin surge were observed. Our data suggested that maternal HFD during lactation might have an additive effect on the onset of the metabolic syndrome in the offspring, irrespective of the nutritional status in utero through the modified leptin surge.
近期报告表明，婴儿早期的营养状况可能会影响儿童后期的健康结局，如肥胖和代谢综合征。因此，我们研究了哺乳期母鼠高脂饮食（HFD）对其后代代谢综合征发病的影响。所有后代均由食用相同或相反饮食的母鼠进行交叉寄养，分为4组：由高脂饮食喂养的母鼠哺乳的高脂饮食喂养母鼠的后代（OHH）、由对照饮食（CD）喂养的母鼠哺乳的高脂饮食喂养母鼠的后代（OHC）、由高脂饮食喂养的母鼠哺乳的对照饮食喂养母鼠的后代（OCH）以及由对照饮食喂养的母鼠哺乳的对照饮食喂养母鼠的后代（OCC）小鼠。我们检测了几个与代谢综合征相关的因素，包括体重、血压、糖耐量和脂肪细胞因子。OHH和OCH小鼠的平均体重分别显著高于OHC和OCC小鼠，收缩压升高。此外，与OHC和OCC小鼠相比，OHH和OCH小鼠的糖耐量分别显著更差。哺乳期母鼠高脂饮食使甘油三酯和瘦素水平显著升高，脂联素水平显著降低，脂肪组织中瘦素和脂联素mRNA表达有类似变化，但无组蛋白修饰。此外，哺乳期母鼠高脂饮食诱导的母体肥胖增加并延长了后代的瘦素激增，且观察到瘦素激增存在性别差异。我们的数据表明，哺乳期母鼠高脂饮食可能对后代代谢综合征的发病有累加效应，无论子宫内的营养状况如何，这一效应通过改变瘦素激增来实现。
