Kurowska E, Szewczuk A
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1988;36(1):7-13.
Non-competitive enzyme-linked immunosorbent assay (ELISA) for rabbit anti-BPTI antibody is described. The antibody was purified by affinity chromatography on Sepharose-BPTI column and then separated into two antibody fractions on Sepharose-trypsin (BPTI) column. The fraction I antibody, not retarded on the second biosorbent column, efficiently inactivated BPTI, but the fraction II antibody, eluted from the column with glycine buffer, pH 2.2 was firmly bound to active BPTI. This proves that BPTI has at least two epitopes.
描述了用于兔抗BPTI抗体的非竞争性酶联免疫吸附测定（ELISA）。该抗体通过在琼脂糖-BPTI柱上进行亲和层析纯化，然后在琼脂糖-胰蛋白酶（BPTI）柱上分离成两个抗体组分。在第二个生物吸附柱上未滞留的I组分抗体能有效灭活BPTI，但用pH 2.2的甘氨酸缓冲液从柱上洗脱的II组分抗体与活性BPTI紧密结合。这证明BPTI至少有两个表位。
