Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA,
Adv Exp Med Biol. 2014;801:23-30. doi: 10.1007/978-1-4614-3209-8_4.
Glutathione S-transferase pi isoform (GSTP1) is an intracellular detoxification enzyme that catalyzes reduction of chemically reactive electrophiles and is a zeaxanthin-binding protein in the human macula. We have previously demonstrated that GSTP1 levels are decreased in human age-related macular degeneration (AMD) retina compared to normal controls (Joshi et al., Invest Ophthalmol Vis Sci, e-abstract, 2009). We also showed that GSTP1 levels parallel survival of human retinal pigment epithelial (RPE) cells exposed to ultraviolet (UV) light, and GSTP1 over-expression protects them against UV light damage (Joshi et al., Invest Ophthalmol Vis Sci, e-abstract, 2010). In the present work, we determined the developmental time course of GSTP1 expression in murine retina and in response to light challenge.
Eyes from BALB/c mice at postnatal day 20, 1 month, and 2 months of age were prepared for retinal protein extraction and cryo sectioning, and GSTP1 levels in the retina were analyzed by Western blot and immunohistochemistry (IHC). Another group of BALB/c mice with the same age ranges was exposed to 1000 lx of white fluorescent light for 24 h, and their retinas were analyzed for GSTP1 expression by Western blot and IHC in a similar manner.
GSTP1 levels in the murine retina increased in ascending order from postnatal day 20, 1 month, and 2 months of age. Moreover, GSTP1 expression in murine retina at postnatal day 20, 1 month, and 2 months of age increased in response to brief light exposure compared to age-matched controls under normal condition.
GSTP1 expression in retina increases with developmental age in mice and accompanies murine retinal maturation. Brief exposure to light induces GSTP1 expression in the murine retina across various developmental ages. GSTP1 induction may be a protective response to light-induced oxidative damage in the murine retina.
谷胱甘肽 S-转移酶 pi 同工酶（GSTP1）是一种细胞内解毒酶，可催化化学活性亲电体的还原，并且是人类黄斑中的玉米黄质结合蛋白。我们之前已经证明，与正常对照相比，人年龄相关性黄斑变性（AMD）视网膜中的 GSTP1 水平降低（Joshi 等人，Invest Ophthalmol Vis Sci，e 摘要，2009）。我们还表明，GSTP1 水平与暴露于紫外线（UV）光的人视网膜色素上皮（RPE）细胞的存活平行，并且 GSTP1 过表达可保护它们免受 UV 光损伤（Joshi 等人，Invest Ophthalmol Vis Sci，e 摘要，2010）。在本工作中，我们确定了 GSTP1 在小鼠视网膜中的发育时间过程以及对光挑战的反应。
从出生后第 20 天、1 个月和 2 个月大的 BALB/c 小鼠中制备视网膜蛋白提取物和冷冻切片，并通过 Western blot 和免疫组织化学（IHC）分析视网膜中的 GSTP1 水平。另一组具有相同年龄范围的 BALB/c 小鼠接受 1000 lx 白色荧光灯照射 24 h，并用 Western blot 和 IHC 以类似方式分析其视网膜中的 GSTP1 表达。
从出生后第 20 天、1 个月和 2 个月大，小鼠视网膜中的 GSTP1 水平呈递增顺序增加。此外，与正常条件下的同龄对照相比，出生后第 20 天、1 个月和 2 个月大的小鼠视网膜中的 GSTP1 表达在短暂光照暴露后增加。
GSTP1 在小鼠视网膜中的表达随年龄的增长而增加，并伴随小鼠视网膜的成熟。短暂暴露于光会在各种发育年龄的小鼠视网膜中诱导 GSTP1 表达。GSTP1 的诱导可能是对小鼠视网膜光诱导氧化损伤的一种保护反应。
