Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany,
Adv Exp Med Biol. 2014;801:145-54. doi: 10.1007/978-1-4614-3209-8_19.
The immortalized RGC-5 cell line has been widely used as a cell culture model to study the neurobiology of retinal ganglion cells (RGCs). The cells were originally introduced as derived from rat RGC showing expression of various neuronal markers, in particular the RGC-characteristic proteins Brn3 and Thy1. Recent data gave rise to concerns regarding the origin and nature of the cells. RGC-5 cells were identified to be of mouse origin and their expression of RGC characteristics was questioned by some laboratories. This article summarizes the available data on the properties of RGC-5, discusses common protocols for their differentiation and is aimed at providing researchers some guidelines on the benefits and limitations of RGC-5 for research.
永生化的 RGC-5 细胞系已被广泛用作研究视网膜神经节细胞 (RGC) 神经生物学的细胞培养模型。这些细胞最初是从表达各种神经元标志物的大鼠 RGC 中引入的，特别是 RGC 特征蛋白 Brn3 和 Thy1。最近的数据引起了人们对细胞起源和性质的关注。RGC-5 细胞被确定为鼠源性，一些实验室对其表达 RGC 特征提出了质疑。本文总结了 RGC-5 细胞特性的现有数据，讨论了其分化的常用方案，并旨在为研究人员提供 RGC-5 在研究中的益处和局限性的一些指导。
