Cheow Wean Sin, Hadinoto Kunn
Division of Chemical and Biomolecular Engineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, N1.2-B2-31, 62 Nanyang Drive, Singapore, 639798, Singapore.
Methods Mol Biol. 2014;1147:227-38. doi: 10.1007/978-1-4939-0467-9_16.
Polymeric nanoparticles are highly attractive as drug delivery vehicles due to their high structural integrity, stability during storage, ease of preparation and functionalization, and controlled release capability. Similarly, lipid-polymer hybrid nanoparticles, which retain the benefits of polymeric nanoparticles plus the enhanced biocompatibility and prolonged circulation time owed to the lipids, have recently emerged as a superior alternative to polymeric nanoparticles. Drug nanoparticle complex prepared by electrostatic interaction of oppositely charged drug and polyelectrolytes represents another type of polymeric nanoparticle. This chapter details the preparation, characterization, and antibiofilm efficacy testing of antibiotic-loaded polymeric and hybrid nanoparticles and antibiotic nanoparticle complex.
聚合物纳米颗粒作为药物递送载体极具吸引力，这归因于其高度的结构完整性、储存期间的稳定性、易于制备和功能化以及控释能力。同样，脂质-聚合物杂化纳米颗粒保留了聚合物纳米颗粒的优点，同时由于脂质而具有增强的生物相容性和延长的循环时间，最近已成为聚合物纳米颗粒的一种更优替代物。通过带相反电荷的药物与聚电解质的静电相互作用制备的药物纳米颗粒复合物代表了另一类聚合物纳米颗粒。本章详细介绍了载抗生素聚合物和杂化纳米颗粒以及抗生素纳米颗粒复合物的制备、表征和抗生物膜功效测试。
