Shukla Shailaja, Dass Jasmita, Pujani Mukta
Department of Pathology, Lady Hardinge Medical College and Smt. Sucheta Kriplani Hospital, New Delhi, India.
Department of Pathology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, New Delhi, India.
South Asian J Cancer. 2014 Jan;3(1):48-53. doi: 10.4103/2278-330X.126524.
Persistent high risk human papilloma virus (HPV) infection is probably the best predictor of increased risk of cervical cancer, but expression of certain markers of cell proliferation and apoptosis have been studied. The present study was conducted to evaluate the expression of p53 and bcl2 in premalignant and malignant lesions of cervix and its correlation with HPV type 16 and 18.
The study comprised of 35 cases (including 24 prospective cases and 11 retrospective cases) of premalignant and malignant lesions of the cervix. Slides were stained with Hematoxylin and Eosin and p53, bcl2 (immunohistochemistry), HPV 16 and HPV 18 (in situ hybridization).
p53 positivity was seen in 8/19 (42.1%) cases of cervical intraepithelial neoplasia (CIN) and 8/16 (50%) cases of carcinoma cervix, the difference not significant statistically. The difference in bcl2 expression in CIN versus carcinoma cervix (84.21% vs. 43.75%) was statistically significant (P = 0.030). There was no significant difference between p53 and bcl2 expression and the stage and grade of the tumors. Seven out of 19 cases of CIN (36.84%) were positive for HPV 16/18 infection and 8/16 cases (50%) of carcinoma cervix were HPV positive (P = 0.628).
No significant association was found between HPV 16/18 infection and p53 and bcl2 expression in premalignant and malignant lesions of uterine cervix. Although, bcl2 staining showed a significant difference between CIN and carcinoma cervix, a larger case series is required to assess the association between HPV infection and overexpression of p53 and bcl2 proteins in these lesions.
持续性高危型人乳头瘤病毒（HPV）感染可能是宫颈癌风险增加的最佳预测指标，但某些细胞增殖和凋亡标志物的表达已得到研究。本研究旨在评估p53和bcl2在宫颈癌前病变和恶性病变中的表达及其与16型和18型HPV的相关性。
本研究包括35例宫颈癌前病变和恶性病变（包括24例前瞻性病例和11例回顾性病例）。玻片用苏木精和伊红染色以及p53、bcl2（免疫组织化学）、HPV 16和HPV 18（原位杂交）进行检测。
19例宫颈上皮内瘤变（CIN）中有8例（42.1%）p53呈阳性，16例宫颈癌中有8例（50%）呈阳性，差异无统计学意义。CIN与宫颈癌中bcl2表达的差异（84.21%对43.75%）具有统计学意义（P = 0.030）。p53和bcl2的表达与肿瘤分期和分级之间无显著差异。19例CIN中有7例（36.84%）HPV 16/18感染呈阳性，16例宫颈癌中有8例（50%）HPV呈阳性（P = 0.628）。
在宫颈的癌前病变和恶性病变中，未发现HPV 16/18感染与p53和bcl2表达之间存在显著关联。虽然，bcl2染色显示CIN与宫颈癌之间存在显著差异，但需要更大的病例系列来评估HPV感染与这些病变中p53和bcl2蛋白过表达之间的关联。
