Kim Y D, Tomita J T, Schenck J R
J Immunol Methods. 1978;19(4):309-16. doi: 10.1016/0022-1759(78)90015-7.
A simple and rapid solid-phase radioimmunoassay for the measurement of carcinoembryonic antigen (CEA) levels in sera or plasma is described. The procedure involves perchloric acid (PCA) extraction of the samples, followed by a rapid neutralization of excess acid with alkaline buffer solution. The PCA extracts are assayed uing antibody-coated plastic tubes and radio-labeled anti-CEA antibody as a marker. The assay may be completed in one day. The solid-phase direct-binding assay demonstrated a sensitivity of 0.5 ng CEA/ml plasmin and a marked tolerance to variations in pH and ionic strength of the system. A fair correlation between the CEA levels determined by the solid-phase radioimmunoassay and zirconium phosphate gel method was observed.
本文描述了一种用于测量血清或血浆中癌胚抗原（CEA）水平的简单快速固相放射免疫分析方法。该方法包括用高氯酸（PCA）提取样品，然后用碱性缓冲溶液快速中和过量的酸。PCA提取物使用包被抗体的塑料管和放射性标记的抗CEA抗体作为标记物进行检测。该分析可在一天内完成。固相直接结合分析显示对纤溶酶中CEA的检测灵敏度为0.5 ng/ml，并且对系统pH值和离子强度的变化具有显著耐受性。观察到固相放射免疫分析测定的CEA水平与磷酸锆凝胶法测定的结果具有较好的相关性。
